The role of CD4(+) and CD8(+) T cells on antibody production by murine Peyer's patch cells following mucosal presentation of Actinomyces viscosus


Autoria(s): Sosroseno, W.; Bird, P. S.; Gemmell, E.; Seymour, G. J.
Contribuinte(s)

J. Slots

Data(s)

01/01/2006

Resumo

The aim of this study was to determine the role of CD4 and CD8 cells on specific antibody production by murine Peyer's patch (PP) cells after oral immunization with Actinomyces viscosus in mice. Female DBA/2 mice were orally immunized with three low doses of heat-killed A. viscosus. Sham-immunized mice served as a control group. Mice were depleted of CD4 or CD8 cells by intraperitoneal injection of anti-CD4 or anti-CD8 antibodies daily for 3 days before oral immunization. One week after the last oral immunization, PPs were removed and cell suspensions were cultured with A. viscosus. Specific antibody production in the culture supernatants was assessed by enzyme-linked immunosorbent assay. The results showed that oral immunization with A. viscosus induced a predominant specific immunoglobulin A (IgA) response by PP cells and, to a lesser extent, IgM antibodies. Depletion of CD4 but not CD8 cells suppressed the production of specific antibodies. These results suggest that oral immunization with low doses of A. viscosus may induce the production of specific antibodies by murine PP cells in a CD4-cell-dependent fashion.

Identificador

http://espace.library.uq.edu.au/view/UQ:81653

Idioma(s)

eng

Publicador

Blackwell Munksgaard

Palavras-Chave #Dentistry, Oral Surgery & Medicine #Immunology #Microbiology #Actinomyces Viscosus #Antibody #Cd4 Cells #Cd8 Cells #Mice #Peyer's Patch #Porphyromonas-gingivalis #Oral Tolerance #In-vivo #Responses #Induction #Antigen #Reveals #Spleen #C1 #320899 Dentistry not elsewhere classified #730112 Oro-dental and disorders
Tipo

Journal Article