Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species


Autoria(s): St Pierre, L.; Woods, R.; Earl, S.; Masci, P. P.; Lavin, M. F.
Contribuinte(s)

P. Jolles

Data(s)

01/01/2005

Resumo

Australian terrestrial elapid snakes contain amongst the most potently toxic venoms known. However, despite the well-documented clinical effects of snake bite, little research has focussed on individual venom components at the molecular level. To further characterise the components of Australian elapid venoms, a complementary (cDNA) microarray was produced from the venom gland of the coastal taipan (Oxyuranus scutellatus) and subsequently screened for venom gland-specific transcripts. A number of putative toxin genes were identified, including neurotoxins, phospholipases, a pseudechetoxin-like gene, a venom natriuretic peptide and a nerve growth factor together with other genes involved in cellular maintenance. Venom gland-specific components also included a calglandulin-like protein implicated in the secretion of toxins from the gland into the venom. These toxin transcripts were subsequently identified in seven other related snake species, producing a detailed comparative analysis at the cDNA and protein levels. This study represents the most detailed description to date of the cloning and characterisation of different genes associated with envenomation from Australian snakes.

Identificador

http://espace.library.uq.edu.au/view/UQ:77014

Idioma(s)

eng

Publicador

Birkhaeuser Verlag AG

Palavras-Chave #Biochemistry & Molecular Biology #Cell Biology #Gene Cloning #Australian Elapid #Oxyuranus Scutellatus #Pseudechetoxin #Calglandulin #Phospholipase A(2) #L-amino Acid Oxidase #Amino-acid-sequences #Gated Ion Channels #Potent Presynaptic Neurotoxin #Phospholipase A(2) Enzymes #Notechis-scutatus-scutatus #Pre-synaptic Neurotoxin #Australian King Brown #Ef-hand Protein #Snake-venom #S-scutellatus #C1 #321206 Preventive Medicine #730220 Injury control
Tipo

Journal Article