Peroxisomes are platforms for cytomegalovirus’ evasion from the cellular immune response


Autoria(s): Magalhães, Ana Cristina; Ferreira, Ana Rita; Gomes, Sílvia; Vieira, Marta; Gouveia, Ana; Valença, Isabel; Islinger, Markus; Nascimento, Rute; Schrader, Michael; Kagan, Jonathan C.; Ribeiro, Daniela
Data(s)

08/06/2016

08/06/2016

16/05/2016

Resumo

The human cytomegalovirus developed distinct evasion mechanisms from the cellular antiviral response involving vMIA, a virally-encoded protein that is not only able to prevent cellular apoptosis but also to inhibit signalling downstream from mitochondrial MAVS. vMIA has been shown to localize at mitochondria and to trigger their fragmentation, a phenomenon proven to be essential for the signalling inhibition. Here, we demonstrate that vMIA is also localized at peroxisomes, induces their fragmentation and inhibits the peroxisomal-dependent antiviral signalling pathway. Importantly, we demonstrate that peroxisomal fragmentation is not essential for vMIA to specifically inhibit signalling downstream the peroxisomal MAVS. We also show that vMIA interacts with the cytoplasmic chaperone Pex19, suggesting that the virus has developed a strategy to highjack the peroxisomal membrane proteins' transport machinery. Furthermore, we show that vMIA is able to specifically interact with the peroxisomal MAVS. Our results demonstrate that peroxisomes constitute a platform for evasion of the cellular antiviral response and that the human cytomegalovirus has developed a mechanism by which it is able to specifically evade the peroxisomal MAVS-dependent antiviral signalling.

Fundação para a Ciência e Tecnologia personal fellowships: (SFRH/BPD/77619/2011, SFRH/BPD/103580/2014, SFRH/BD/81223/2011, SFRH/BD/101942/2014); FCT grants: (PTDC-IMI-MIC-0828-2012 - Programa Operacional Temático Factores de Competitividade” (COMPETE) of “Quadro Comunitário de Apoio III”); Fundo Comunitário Europeu (FEDER); BiMED - Institute for Biomedicine grant: (UID/BIM/04501/2013).

Identificador

Magalhães, A. C. et al. Peroxisomes are platforms for cytomegalovirus’ evasion from the cellular immune response. Sci. Rep. 6, 26028; doi: 10.1038/srep26028 (2016).

http://hdl.handle.net/10400.7/631

10.1038/srep26028

Idioma(s)

eng

Publicador

Nature Publishing Group

Relação

http://www.nature.com/articles/srep26028

Direitos

openAccess

http://creativecommons.org/licenses/by/4.0/

Palavras-Chave #Immune evasion #Peroxisomes
Tipo

article