Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.
Data(s) |
01/07/2012
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Resumo |
BACKGROUND: Pru p 3 is the major peach allergen and the most frequent cause of food allergy in adults in the Mediterranean area. Although its allergenicity is well characterized, its ability to generate a T-cell response is not completely known. OBJECTIVE: To investigate the influence of Pru p 3 allergen on dendritic cell (DC) maturation and specific T-cell response (T(H)1/T(H)2) in peach allergic patients. METHODS: Peach allergic patients (n = 11) and tolerant controls (n = 14) were included in the study. Monocyte-derived DC maturation after incubation with Pru p 3 was evaluated by the increase of maturational markers (CD80, CD86, and CD83) by flow cytometry. Lymphocyte proliferation was evaluated by coculturing monocyte-derived DCs and 5,6-carboxyfluorescein diacetate N-succinimidyl ester-stained lymphocytes with different concentrations of Pru p 3 (25, 10, and 1 ?g/mL) by flow cytometry and cytokine production. RESULTS: Pru p 3 induced a significant increase in the CD80, CD86, and CD83 expression on stimulated DCs from patients compared with controls. The lymphocyte proliferative response after Pru p 3 stimulation was also significantly higher along with an increase in interleukin 8 in patients compared with tolerant controls. CONCLUSION: Pru p 3 allergen induces changes in DC maturational status mainly in peach allergic patients. An increase in lymphocyte proliferative response accompanied with a different cytokine pattern was also observed compared with healthy controls. |
Formato |
application/pdf |
Identificador | |
Idioma(s) |
eng |
Publicador |
E.T.S.I. Agrónomos (UPM) |
Relação |
http://oa.upm.es/16235/1/INVE_MEM_2012_132904.pdf http://www.annallergy.org/article/S1081-1206(12)00391-2/abstract info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anai.2012.05.017 |
Direitos |
http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/restrictedAccess |
Fonte |
Annals of Allergy Asthma & Immunology, ISSN 1081-1206, 2012-07, Vol. 109, No. 1 |
Palavras-Chave | #Biología |
Tipo |
info:eu-repo/semantics/article Artículo PeerReviewed |