Seawater carbonate chemistry, pigments and proteins during experiments with phytoplankton, 2010


Autoria(s): Hopkinson, Brian A; Xu, Yan; Shi, Dalin; McGinn, Patrick J; Morel, Francois M M
Data(s)

08/03/2010

Resumo

In the high-nutrient, low-chlorophyll waters of the Gulf of Alaska, microcosm manipulation experiments were used to assess the effect of CO2 on growth and primary production under iron-limited and iron-replete conditions. As expected, iron had a strong effect on growth and photosynthesis. A modest and variable stimulation of growth and biomass production by CO2 (high CO2: 77-122 Pa; low CO2: 11-17 Pa) was observed under both iron-replete and iron-limited conditions, though near the limit of precision of our measurements in slow-growing low-iron experiments. Physiological acclimations responsible for the changes in growth were assessed. Under iron-limited conditions, growth stimulation at high CO2 appeared to result from an increase in photosynthetic efficiency, which we attribute to energy savings from down-regulation of the carbon concentrating mechanisms. In some cases, iron-rich photosynthetic proteins (PsbA, PsaC, and cytochrome b6) were down-regulated at elevated CO2in iron-limited controls. Under iron-replete conditions, there was an increase in growth rate and biomass at high CO2 in some experiments. This increase was unexpectedly supported by reductions in cellular carbon loss, most likely decreased respiration. We speculate that this effect may be due to acclimation to decreased pH rather than high CO2. The variability in responses to CO2 among experiments did not appear to be caused by differences in phytoplankton community structure and may reflect the sensitivity of the net response of phytoplankton to antagonistic effects of the several parameters that co-vary with CO2.

Formato

text/tab-separated-values, 1001 data points

Identificador

https://doi.pangaea.de/10.1594/PANGAEA.758071

doi:10.1594/PANGAEA.758071

Idioma(s)

en

Publicador

PANGAEA

Direitos

CC-BY: Creative Commons Attribution 3.0 Unported

Access constraints: unrestricted

Fonte

Supplement to: Hopkinson, Brian A; Xu, Yan; Shi, Dalin; McGinn, Patrick J; Morel, Francois M M (2010): The effect of CO2 on the photosynthetic physiology of phytoplankton in the Gulf of Alaska. Limnology and Oceanography, 55(5), 2011-2024, doi:10.4319/lo.2010.55.5.2011

Palavras-Chave #19-Butanoyloxyfucoxanthin; 19-Butanoyloxyfucoxanthin, standard deviation; 19-Hexanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin, standard deviation; abundance; Alkalinity, total; Aragonite saturation state; Bicarbonate ion; BRcommunity; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyll a; Chlorophyll a, standard deviation; Chlorophyll b; Chlorophyll b, standard deviation; Colorimetry; community composition; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; field; Fluorometry; Fucoxanthin; Fucoxanthin, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); growth; High Performance Liquid Chromatography (HPLC); molecular biology; multiple factors; Nitrate; North Pacific; nutrients; OA-ICC; Ocean Acidification International Coordination Centre; other process; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; pH meter (Oakton); Phosphate; Photosynthetic protein cytochrome b6f; Photosynthetic protein cytochrome b6f standard deviation; Photosynthetic protein PsbA; Photosynthetic protein PsbA standard deviation; Photosynthetic protein PsbC; Photosynthetic protein PsbC standard deviation; primary production; Salinity; see reference(s); Silicon; Temperature, water; Time, incubation
Tipo

Dataset