Expression and biological activities of bovine interleukin 4: effects of recombinant bovine interleukin 4 on T cell proliferation and B cell differentiation and proliferation in vitro


Autoria(s): Estes, D M; Hirano, A; Heussler, Volker; Dobbelaere, D A; Brown, W C
Data(s)

01/07/1995

Resumo

Interleukin 4 (IL-4) is a pleotropic cytokine affecting a wide range of cell types in both the mouse and the human. These activities include regulation of the growth and differentiation of both T and B lymphocytes. The activities of IL-4 in nonprimate, nonmurine systems are not well established. Herein, we demonstrate in the bovine system that IL-4 upregulates production of IgM, IgG1, and IgE in the presence of a variety of costimulators including anti-IgM, Staphylococcus aureus cowan strain I, and pokeweed mitogen. IgE responses are potentiated by the addition of IL-2 to IL-4. Culture of bovine B lymphocytes with IL-4 in the absence of additional costimulators resulted in the increased surface expression of CD23 (low-affinity Fc epsilon RII), IgM, IL-2R, and MHC class II in a dose-dependent manner. IL-4 alone increased basal levels of proliferation of bulk peripheral blood mononuclear cells but in the presence of Con A inhibited proliferation. In contrast to the activities of IL-4 in the murine system, proliferation of TH1- and TH2-like clones was inhibited in a dose-dependent manner as assessed by antigen-or IL-2-driven in vitro proliferative responses. These observations are consistent with the role of IL-4 as a key player in regulation of both T and B cell responses.

Formato

application/pdf

Identificador

http://boris.unibe.ch/83750/1/Biological.pdf

Estes, D M; Hirano, A; Heussler, Volker; Dobbelaere, D A; Brown, W C (1995). Expression and biological activities of bovine interleukin 4: effects of recombinant bovine interleukin 4 on T cell proliferation and B cell differentiation and proliferation in vitro. Cellular immunology, 163(2), pp. 268-279. Elsevier 10.1006/cimm.1995.1126 <http://dx.doi.org/10.1006/cimm.1995.1126>

doi:10.7892/boris.83750

info:doi:10.1006/cimm.1995.1126

info:pmid:7606798

urn:issn:0008-8749

Idioma(s)

eng

Publicador

Elsevier

Relação

http://boris.unibe.ch/83750/

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Estes, D M; Hirano, A; Heussler, Volker; Dobbelaere, D A; Brown, W C (1995). Expression and biological activities of bovine interleukin 4: effects of recombinant bovine interleukin 4 on T cell proliferation and B cell differentiation and proliferation in vitro. Cellular immunology, 163(2), pp. 268-279. Elsevier 10.1006/cimm.1995.1126 <http://dx.doi.org/10.1006/cimm.1995.1126>

Palavras-Chave #570 Life sciences; biology
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed