The Host Nonsense-Mediated mRNA Decay Pathway Restricts Mammalian RNA Virus Replication


Autoria(s): Balistreri, Giuseppe; Horvath, Peter; Schweingruber, Christoph; Zünd, David; McInerney, Gerald; Merits, Andres; Mühlemann, Oliver; Azzalin, Claus; Helenius, Ari
Data(s)

10/09/2014

Resumo

In addition to classically defined immune mechanisms, cell-intrinsic processes can restrict virus infection and have shaped virus evolution. The details of this virus-host interaction are still emerging. Following a genome-wide siRNA screen for host factors affecting replication of Semliki Forest virus (SFV), a positive-strand RNA (+RNA) virus, we found that depletion of nonsense-mediated mRNA decay (NMD) pathway components Upf1, Smg5, and Smg7 led to increased levels of viral proteins and RNA and higher titers of released virus. The inhibitory effect of NMD was stronger when virus replication efficiency was impaired by mutations or deletions in the replicase proteins. Consequently, depletion of NMD components resulted in a more than 20-fold increase in production of these attenuated viruses. These findings indicate that a cellular mRNA quality control mechanism serves as an intrinsic barrier to the translation of early viral proteins and the amplification of +RNA viruses in animal cells.

Formato

application/pdf

Identificador

http://boris.unibe.ch/58739/1/Balistreri%202014%20Cell%20Host%20Microbe.pdf

Balistreri, Giuseppe; Horvath, Peter; Schweingruber, Christoph; Zünd, David; McInerney, Gerald; Merits, Andres; Mühlemann, Oliver; Azzalin, Claus; Helenius, Ari (2014). The Host Nonsense-Mediated mRNA Decay Pathway Restricts Mammalian RNA Virus Replication. Cell host & microbe, 16(3), pp. 403-411. Cell Press 10.1016/j.chom.2014.08.007 <http://dx.doi.org/10.1016/j.chom.2014.08.007>

doi:10.7892/boris.58739

info:doi:10.1016/j.chom.2014.08.007

urn:issn:1931-3128

Idioma(s)

eng

Publicador

Cell Press

Relação

http://boris.unibe.ch/58739/

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Balistreri, Giuseppe; Horvath, Peter; Schweingruber, Christoph; Zünd, David; McInerney, Gerald; Merits, Andres; Mühlemann, Oliver; Azzalin, Claus; Helenius, Ari (2014). The Host Nonsense-Mediated mRNA Decay Pathway Restricts Mammalian RNA Virus Replication. Cell host & microbe, 16(3), pp. 403-411. Cell Press 10.1016/j.chom.2014.08.007 <http://dx.doi.org/10.1016/j.chom.2014.08.007>

Palavras-Chave #570 Life sciences; biology #540 Chemistry
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed