Isolation and genotyping of Toxoplasma gondii causing fatal systemic toxoplasmosis in an immunocompetent 10-year-old cat
Data(s) |
2011
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Resumo |
A 10-year-old male, neutered domestic shorthair cat was presented with fever, anorexia, vomiting, and diarrhea. Serologic testing for Feline immunodeficiency virus and Feline leukemia virus were negative. Fine-needle aspirates of mesenteric lymph nodes revealed the presence of banana-shaped apicomplexan parasites. The cat died after 4 days of hospitalization. Postmortem polymerase chain reaction (PCR) analysis confirmed the presence of Toxoplasma gondii in all examined organs. Parasites were ex vivo isolated in outbred mice and subsequently transferred into cell culture. Genotyping, using genetic markers for SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico for PCR-restriction fragment length polymorphism, revealed infection with type II T. gondii displaying type II alleles at all loci except Apico, which exhibited a type I allele. This is the most frequently identified genotype among cats acting as definitive hosts in central Europe, but to the authors' knowledge, it has never been associated with systemic toxoplasmosis in an adult, immunocompetent cat. |
Formato |
application/pdf |
Identificador |
http://boris.unibe.ch/12043/1/Spycher_2011.pdf Spycher, A.; Geigy, C.; Howard, J.; Posthaus, Horst; Gendron, Karine; Gottstein, Bruno; Debache, K.; Herrmann, D.C.; Schares, G.; Frey, Caroline (2011). Isolation and genotyping of Toxoplasma gondii causing fatal systemic toxoplasmosis in an immunocompetent 10-year-old cat. Journal of veterinary diagnostic investigation, 23(1), pp. 104-8. Columbia, Mo.: American Association of Veterinary Laboratory Diagnosticians AAVLD 10.1177/104063871102300117 <http://dx.doi.org/10.1177/104063871102300117> doi:10.7892/boris.12043 info:doi:10.1177/104063871102300117 info:pmid:21217037 urn:issn:1040-6387 |
Idioma(s) |
eng |
Publicador |
American Association of Veterinary Laboratory Diagnosticians AAVLD |
Relação |
http://boris.unibe.ch/12043/ |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Spycher, A.; Geigy, C.; Howard, J.; Posthaus, Horst; Gendron, Karine; Gottstein, Bruno; Debache, K.; Herrmann, D.C.; Schares, G.; Frey, Caroline (2011). Isolation and genotyping of Toxoplasma gondii causing fatal systemic toxoplasmosis in an immunocompetent 10-year-old cat. Journal of veterinary diagnostic investigation, 23(1), pp. 104-8. Columbia, Mo.: American Association of Veterinary Laboratory Diagnosticians AAVLD 10.1177/104063871102300117 <http://dx.doi.org/10.1177/104063871102300117> |
Palavras-Chave | #630 Agriculture |
Tipo |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed |