Insights in the maturation of pathogenic bacteria vaccine candidates using mass spectrometry based approaches


Autoria(s): Donnarumma, Danilo
Contribuinte(s)

Scarlato, Vincenzo

Norais, Nathalie

Data(s)

27/04/2012

Resumo

The study of the maturation process that occurs to a protein is of pivotal importance for the understanding of its function. This is true also in the vaccine field but in this case is also important to evaluate if inappropriate protein conformation and maturation play roles in the impairment of the functional immunogenicity of protein vaccines. Mass spectrometry (MS) is the method of choice for the study of the maturation process since each modification that occurs during the maturation will lead to a change in the mass of the entire protein. Therefore the aim of my thesis is the development of mass spectrometry-based approaches to study the maturation of proteins and the application of these methods to proteic vaccine candidates. The thesis is divided in two main parts. In the first part, I focused my attention on the study of the maturation of different vaccine candidates using native mass spectrometry. The analyses in this case have been performed using recombinant proteins produced in E. coli. In the second part I applied different MS strategies for the identification of unknown PTMs on pathogenic bacteria surface proteins since modified surface proteins are now considered for vaccine candidate selection.

Formato

application/pdf

Identificador

http://amsdottorato.unibo.it/4829/1/INSIGHTS_IN_THE_MATURATION_OF_PATHOGENIC_BACTERIA_VACCINE_CANDIDATES_USING_MASS_SPECTROMETRY_BASED_APPROACHES.pdf

urn:nbn:it:unibo-4092

Donnarumma, Danilo (2012) Insights in the maturation of pathogenic bacteria vaccine candidates using mass spectrometry based approaches, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Biologia cellulare, molecolare e industriale/cellular, molecular and industrial biology: progetto n. 2 Biologia funzionale dei sistemi cellulari e molecolari <http://amsdottorato.unibo.it/view/dottorati/DOT453/>, 24 Ciclo. DOI 10.6092/unibo/amsdottorato/4829.

Idioma(s)

en

Publicador

Alma Mater Studiorum - Università di Bologna

Relação

http://amsdottorato.unibo.it/4829/

Direitos

info:eu-repo/semantics/openAccess

Palavras-Chave #BIO/11 Biologia molecolare
Tipo

Tesi di dottorato

NonPeerReviewed