Multivariate spectroscopic methods for the analysis of solutions


Autoria(s): Wiberg, Kent
Data(s)

2004

Resumo

In this thesis some multivariate spectroscopic methods for the analysis of solutions are proposed. Spectroscopy and multivariate data analysis form a powerful combination for obtaining both quantitative and qualitative information and it is shown how spectroscopic techniques in combination with chemometric data evaluation can be used to obtain rapid, simple and efficient analytical methods. These spectroscopic methods consisting of spectroscopic analysis, a high level of automation and chemometric data evaluation can lead to analytical methods with a high analytical capacity, and for these methods, the term high-capacity analysis (HCA) is suggested. It is further shown how chemometric evaluation of the multivariate data in chromatographic analyses decreases the need for baseline separation. The thesis is based on six papers and the chemometric tools used are experimental design, principal component analysis (PCA), soft independent modelling of class analogy (SIMCA), partial least squares regression (PLS) and parallel factor analysis (PARAFAC). The analytical techniques utilised are scanning ultraviolet-visible (UV-Vis) spectroscopy, diode array detection (DAD) used in non-column chromatographic diode array UV spectroscopy, high-performance liquid chromatography with diode array detection (HPLC-DAD) and fluorescence spectroscopy. The methods proposed are exemplified in the analysis of pharmaceutical solutions and serum proteins. In Paper I a method is proposed for the determination of the content and identity of the active compound in pharmaceutical solutions by means of UV-Vis spectroscopy, orthogonal signal correction and multivariate calibration with PLS and SIMCA classification. Paper II proposes a new method for the rapid determination of pharmaceutical solutions by the use of non-column chromatographic diode array UV spectroscopy, i.e. a conventional HPLC-DAD system without any chromatographic column connected. In Paper III an investigation is made of the ability of a control sample, of known content and identity to diagnose and correct errors in multivariate predictions something that together with use of multivariate residuals can make it possible to use the same calibration model over time. In Paper IV a method is proposed for simultaneous determination of serum proteins with fluorescence spectroscopy and multivariate calibration. Paper V proposes a method for the determination of chromatographic peak purity by means of PCA of HPLC-DAD data. In Paper VI PARAFAC is applied for the decomposition of DAD data of some partially separated peaks into the pure chromatographic, spectral and concentration profiles.

Formato

application/pdf

Identificador

http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-110

urn:isbn:91-7265-789-8

Idioma(s)

eng

Publicador

Stockholms universitet, Institutionen för analytisk kemi

Stockholm : Institutionen för analytisk kemi

Direitos

info:eu-repo/semantics/openAccess

Palavras-Chave #Chemometrics #UV-Vis spectroscopy #Multivariate calibration #Lidocaine #Identity #Content #PLS #SIMCA #Non-column #Diode array UV spectroscopy #DAD #Control sample #High Capacity Analysis (HCA) #Fluorescence spectroscopy #Albumin #Immunoglobulin G #HPLC-DAD #Prilocaine #Peak purity determination #PCA #PARAFAC #Partial separation #Curve resolution #Analytical chemistry #Analytisk kemi
Tipo

Doctoral thesis, comprehensive summary

info:eu-repo/semantics/doctoralThesis

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