Phenotypic and genotypic features of Aggregatibacter actinomycetemcomitans isolated from patients with periodontal disease
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
24/03/2014
24/03/2014
01/04/2013
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Resumo |
Aggregatibacter actinomycetemcomitans is strongly implicated in the pathogenesis of periodontitis. In this study, the phenotypic and genotypic features of A. actinomycetemcomitans and the presence of genes involved in toxicity were determined. Sixty-five patients with periodontal pocket and 48 healthy subjects were evaluated. Biotyping, adherence and invasion, neuraminidase and biofilm production, presence of capsule and fimbria, as well as the presence of flp-1, apaH, ltx, and cdt genes were determined. Biotype II was the most prevalent. Sixty-six strains were adherent and 33 of them were able to invade KB cells. Sixty strains produced neuraminidase, and 55 strains biofilms. Strains showed capsule but not fimbriae. Forty-six strains were cytotoxic, and most strains harbored the apaH and flp-1 genes. LTX promoter and the ltxA gene were observed in all strains from periodontal patients. The cdtA gene was observed in 50 (71.4%) strains, cdtB in 48 (68.6%) strains, cdtC in 60 (85.7%), and cdtABC in 40 (57.1%) strains. The presence of A. actinomycetemcomitans harboring the cdtC gene from healthy subjects may represent a transitory microorganism in the oral microbiota. More studies are necessary to understand the real role of this microorganism in the pathogenesis of periodontal disease |
Identificador |
Diagnostic Microbiology and Infectious Disease, New York, v.75, n.4, p.366-372, 2013 http://www.producao.usp.br/handle/BDPI/44263 10.1016/j.diagmicrobio.2012.12.013 |
Idioma(s) |
eng |
Publicador |
Elsevier Biomedical New York |
Relação |
Diagnostic Microbiology and Infectious Disease |
Direitos |
restrictedAccess Elsevier Inc |
Palavras-Chave | #Adherence #Invasion #Periodontal disease #Aggregatibacter actinomycetemcomitans #Periodontite |
Tipo |
article original article publishedVersion |