Simultaneous quantification of lycopene, β-carotene, retinol and α -tocopherol in plasma after a simple extraction procedure: stability study and application to human volunteers


Autoria(s): Charão, Mariele F.; Moro, Angela M.; Brucker, Natália; Bulcão, Rachel P.; Baierle, Marília; Freitas, Fernando; Durgante, Juliano; Nascimento, Sabrina; Bubols, Guilherme B.; Saldiva, Paulo H.; Bohrer, Denise; Garcia, Solange C.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

04/11/2013

04/11/2013

2012

Resumo

A method for the simultaneous quantification of lycopene, β-carotene, retinol and α-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20°C for three months for stability study. The methodology was applied to samples from painters and individuals not exposed to paints (n = 75). The assay was linear for all vitamins (r > 0.99). Intra- and inter-run precisions were obtained with coefficient of variation smaller than 5%. The accuracies ranged from 0.29 to -5.80% and recoveries between 92.73 and 101.97%. Plasma samples and extracted supernatants were stable for 60 days at -20°C. A significant decrease of lycopene, β-carotene and retinol concentrations in plasma from exposed individuals compared to non-exposed individuals (p < 0.05) was observed. The method is simple, reproducible, precise, accurate and sensitive, and can be routinely utilized in clinical laboratories.

Identificador

J. Braz. Chem. Soc.,v.23,n.8,p.1441-1449,2012

0103-5053

http://www.producao.usp.br/handle/BDPI/39233

10.1590/S0103-50532012005000001

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000800006&lng=en&nrm=iso&tlng=en

http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-50532012000800006&lng=en&nrm=iso&tlng=en

http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0103-50532012000800006&lng=en&nrm=iso&tlng=en

Idioma(s)

eng

Publicador

Sociedade Brasileira de Química

Relação

Journal of the Brazilian Chemical Society

Direitos

openAccess

Palavras-Chave #retinol #α-tocopherol #lycopene #β-carotene #HPLC #liquid-liquid extraction
Tipo

article

original article