A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
04/11/2013
04/11/2013
2012
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Resumo |
To assess topical delivery studies of glycoalkaloids, an analytical method by HPLC-UV was developed and validated for the determination of solasonine (SN) and solamargine (SM) in different skin layers, as well as in a topical formulation. The method was linear within the ranges 0.86 to 990.00 µg/mL for SN and 1.74 to 1000.00 µg/mL for SM (r = 0.9996). Moreover, the recoveries for both glycoalkaloids were higher than 88.94 and 93.23% from skin samples and topical formulation, respectively. The method developed is reliable and suitable for topical delivery skin studies and for determining the content of SN and SM in topical formulations. |
Identificador |
Quím. Nova,v.35,n.11,p.2312-2316,2012 0100-4042 http://www.producao.usp.br/handle/BDPI/38626 10.1590/S0100-40422012001100038 http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-40422012001100038&lng=en&nrm=iso&tlng=en |
Idioma(s) |
eng |
Publicador |
Sociedade Brasileira de Química |
Relação |
Química Nova |
Direitos |
openAccess |
Palavras-Chave | #solasonine #solamargine #validation |
Tipo |
article original article |