Evidence supporting a protective role for Th9 and Th22 cytokines in human and experimental periapical lesions
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/09/2013
19/09/2013
01/01/2013
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Resumo |
Introduction: The development of periapical granulomas is dependent on the host response and involves Th1, Th2, Th17, and Treg-related cytokines. The discovery of new Th9 and Th22 subsets, with important immunomodulatory roles mediated by interleukin (IL)-9 and IL-22, respectively, emphasizes the need for reevaluation of current cytokine paradigms in context of periapical lesions. We investigated the expression of IL-9 and IL-22 in active and stable human granulomas and throughout experimental lesion development in mice. Methods: Periapical granulomas (N = 83) and control specimens (N = 24) were evaluated regarding the expression of IL-9 and IL-22 via realtime polymerase chain reaction. Experimental periapical lesions were induced in mice (pulp exposure and bacterial inoculation) and the lesions evolution correlation with IL-9 and IL-22 expression kinetics was evaluated. Results: IL-9 and IL-22 mRNA expression was higher in periapical lesions than in control samples; higher levels of IL-9 and IL-22 were observed in inactive than in active lesions. In the experimental lesions model, increasing levels of IL-9 and IL-22 mRNA were detected in the lesions, and inverse correlations were found between IL-9 and IL-22 and the increase of lesion area in the different time point intervals. Conclusions: Our results suggest that Th9 and Th22 pathways may contribute to human and experimental periapical lesion stability |
Identificador |
Journal of Endodontics, Chicago, v. 39, n. 1, p. 83-87, Jan. 2013 0099-2399 http://www.producao.usp.br/handle/BDPI/33471 10.1016/j.joen.2012.10.015 |
Idioma(s) |
eng |
Publicador |
Elsevier Chicago |
Relação |
Journal of Endodontics |
Direitos |
restrictedAccess Elsevier |
Palavras-Chave | #Apical granulomas #Cytokines #immunoregulation #qPCR #GRANULOMA PERIAPICAL #CITOCINAS #IMUNOLOGIA |
Tipo |
article original article publishedVersion |