Expression, purification and structural analysis of the Pyrococcus abyssi RNA binding protein PAB1135
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
26/08/2013
26/08/2013
2010
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| Resumo |
Abstract Background The gene coding for the uncharacterized protein PAB1135 in the archaeon Pyrococcus abyssi is in the same operon as the ribonuclease P (RNase P) subunit Rpp30. Findings Here we report the expression, purification and structural analysis of PAB1135. We analyzed the interaction of PAB1135 with RNA and show that it binds efficiently double-stranded RNAs in a non-sequence specific manner. We also performed molecular modeling of the PAB1135 structure using the crystal structure of the protein Af2318 from Archaeoglobus fulgidus (2OGK) as the template. Conclusions Comparison of this model has lead to the identification of a region in PAB1135 that could be involved in recognizing double-stranded RNA. This work was supported by a FAPESP grant (07/57096-9 to C.C.O.). J.S.L. was recipient of a FAPESP fellowship. This work was supported by a FAPESP grant (07/570969 to C.C.O.). J.S.L. was recipient of a FAPESP fellowship. |
| Identificador |
1756-0500 http://www.producao.usp.br/handle/BDPI/33169 10.1186/1756-0500-3-97 |
| Idioma(s) |
eng |
| Relação |
BMC Research Notes |
| Direitos |
openAccess Oliveira et al; licensee BioMed Central Ltd. - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Tipo |
article original article |
