IFN-gamma Plays a Unique Role in Protection against Low Virulent Trypanosoma cruzi Strain
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
07/11/2013
07/11/2013
2012
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Resumo |
Background: T. cruzi strains have been divided into six discrete typing units (DTUs) according to their genetic background. These groups are designated T. cruzi I to VI. In this context, amastigotes from G strain (T. cruzi I) are highly infective in vitro and show no parasitemia in vivo. Here we aimed to understand why amastigotes from G strain are highly infective in vitro and do not contribute for a patent in vivo infection. Methodology/Principal Findings: Our in vitro studies demonstrated the first evidence that IFN-gamma would be associated to the low virulence of G strain in vivo. After intraperitoneal amastigotes inoculation in wild-type and knockout mice for TNF-alpha, Nod2, Myd88, iNOS, IL-12p40, IL-18, CD4, CD8 and IFN-gamma we found that the latter is crucial for controlling infection by G strain amastigotes. Conclusions/Significance: Our results showed that amastigotes from G strain are highly infective in vitro but did not contribute for a patent infection in vivo due to its susceptibility to IFN-gamma production by host immune cells. These data are useful to understand the mechanisms underlying the contrasting behavior of different T. cruzi groups for in vitro and in vivo infection. CAPES [3038.005295/2011-40] CAPES FAPEMIG FAPEMIG [APQ-00621-11] CNPq CNPq FAPESP FAPESP [10-50959-4] |
Identificador |
PLOS NEGLECTED TROPICAL DISEASES, SAN FRANCISCO, v. 6, n. 4, supl. 4, Part 1-2, pp. 456-U150, APR, 2012 1935-2735 http://www.producao.usp.br/handle/BDPI/42792 10.1371/journal.pntd.0001598 |
Idioma(s) |
eng |
Publicador |
PUBLIC LIBRARY SCIENCE SAN FRANCISCO |
Relação |
PLOS NEGLECTED TROPICAL DISEASES |
Direitos |
openAccess Copyright PUBLIC LIBRARY SCIENCE |
Palavras-Chave | #CHAGAS-DISEASE #NITRIC-OXIDE #METACYCLIC TRYPOMASTIGOTES #MICE DEFICIENT #LINEAGE-I #INFECTION #MACROPHAGES #PREDOMINANCE #RESISTANCE #MECHANISM #INFECTIOUS DISEASES #PARASITOLOGY #TROPICAL MEDICINE |
Tipo |
article original article publishedVersion |