Culture medium of diluted skimmed milk for the production of nisin in batch cultivations
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
05/11/2013
05/11/2013
2012
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Resumo |
Nisin is a promising alternative to chemical preservatives for use as a natural biopreservative in foods. This bacteriocin has also potential biomedical applications. Lactic acid bacteria are commonly cultivated in expensive standard complex media. We have evaluated the cell growth and nisin production of Lactococcus lactis in a low-cost natural medium consisting of diluted skimmed milk in a 2-L bioreactor. The assays were performed at 30 degrees C for 56 h, at varying agitation speeds and airflow rates: (1) 200 rpm (no airflow, and airflow at 0.5, 1.0 and 2.0 L/min); (2) 100 rpm (no airflow, and airflow at 0.5 L/min). Nisin activity was evaluated using agar diffusion assays. The highest nisin concentration, 49.88 mg/L (3.3 log AU/mL or 1,995.29 AU/mL), was obtained at 16 h of culture, 200 rpm and no airflow (k(L)a = 5.29 x 10(-3)). These results show that a cultivation medium composed of diluted skimmed milk supports cell growth to facilitate nisin biosynthesis. Coordination for the Improvement of Higher Level Personnel (Capes) Coordination for the Improvement of Higher Level Personnel (CAPES) State of Sao Paulo Research Foundation (FAPESP) State of Sao Paulo Research Foundation (Fapesp) National Council for Scientific and Technological Development (CNPq) National Council for Scientific and Technological Development (CNPq) |
Identificador |
ANNALS OF MICROBIOLOGY, NEW YORK, v. 62, n. 1, p. 419-426, MAR, 2012 1590-4261 http://www.producao.usp.br/handle/BDPI/41945 10.1007/s13213-011-0278-6 |
Idioma(s) |
eng |
Publicador |
SPRINGER NEW YORK |
Relação |
Annals of Microbiology |
Direitos |
closedAccess Copyright SPRINGER |
Palavras-Chave | #LACTOCOCCUS LACTIS #MICROAEROPHILIC #PRESERVATIVE #LACTIC ACID BACTERIA #BACTERIOCIN #BIOREACTOR #MUSSEL-PROCESSING WASTES #LACTIC-ACID PRODUCTION #LACTOCOCCUS-LACTIS #CHEESE WHEY #BACTERIA #GROWTH #FERMENTATION #TEMPERATURE #MASTITIS #CECT-539 #BIOTECHNOLOGY & APPLIED MICROBIOLOGY #MICROBIOLOGY |
Tipo |
article original article publishedVersion |