Effects of Natural Radiation, Photosynthetically Active Radiation and Artificial Ultraviolet Radiation-B on the Chloroplast Organization and Metabolism of Porphyra acanthophora var. brasiliensis (Rhodophyta, Bangiales)


Autoria(s): Bouzon, Zenilda L.; Chow, Fungyi; Zitta, Carmen S.; dos Santos, Rodrigo W.; Ouriques, Luciane C.; Felix, Marthiellen R. de L.; Osorio, Luz K. P.; Gouveia, Claudiane; Martins, Roberta de Paula; Latini, Alexandra; Ramlov, Fernanda; Maraschin, Marcelo; Schmidt, Eder C.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

06/11/2013

06/11/2013

2012

Resumo

We undertook a study of Porphyra acanthophora var. brasiliensis to determine its responses under ambient conditions, photosynthetically active radiation (PAR), and PAR+UVBR (ultraviolet radiation-B) treatment, focusing on changes in ultrastructure, and cytochemistry. Accordingly, control ambient samples were collected in the field, and two different treatments were performed in the laboratory. Plants were exposed to PAR at 60 mu mol photons m(-2) s(-1) and PAR+UVBR at 0.35 W m(-2) for 3 h per day during 21 days of in vitro cultivation. Confocal laser scanning microscopy analysis of the vegetative cells showed single stellate chloroplast in ambient and PAR samples, but in PAR+UVBR-exposed plants, the chloroplast showed alterations in the number and form of arms. Under PAR+UVBR treatment, the thylakoids of the chloroplasts were disrupted, and an increase in the number of plastoglobuli was observed, in addition to mitochondria, which appeared with irregular, disrupted morphology compared to ambient and PAR samples. After UVBR exposure, the formation of carpospores was also observed. Plants under ambient conditions, as well as those treated with PAR and PAR+UVBR, all showed different concentrations of enzymatic response, including glutathione peroxidase and reductase activity. In summary, the present study demonstrates that P. acanthophora var. brasiliensis shows the activation of distinct mechanisms against natural radiation, PAR and PAR+UVBR.

CAPES

CAPES

Identificador

MICROSCOPY AND MICROANALYSIS, NEW YORK, v. 18, n. 6, supl. 2, Part 3, pp. 1467-1479, DEC, 2012

1431-9276

http://www.producao.usp.br/handle/BDPI/42202

10.1017/S1431927612013359

http://dx.doi.org/10.1017/S1431927612013359

Idioma(s)

eng

Publicador

CAMBRIDGE UNIV PRESS

NEW YORK

Relação

MICROSCOPY AND MICROANALYSIS

Direitos

closedAccess

Copyright CAMBRIDGE UNIV PRESS

Palavras-Chave #CARPOSPORES #CHLOROPLASTS #ENZYMATIC RESPONSE #PHOTOSYNTHETIC PIGMENTS #PORPHYRA ACANTHOPHORA VAR. BRASILIENSIS #ULTRAVIOLET RADIATION-B #KAPPAPHYCUS-ALVAREZII RHODOPHYTA #UV-B #GRACILARIA-TIKVAHIAE #SPITSBERGEN NORWAY #PALMARIA-PALMATA #GIGARTINALES #MACROALGAE #RESPONSES #ULTRASTRUCTURE #EXPOSURE #MATERIALS SCIENCE, MULTIDISCIPLINARY #MICROSCOPY
Tipo

article

original article

publishedVersion