Molecular and phylogenetic analyses of human Parvovirus B19 isolated from Brazilian patients with sickle cell disease and beta-thalassemia major and healthy blood donors
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
---|---|
Data(s) |
19/09/2013
19/09/2013
2012
|
Resumo |
Human Parvovirus B19 (B19V) is a recognized cause of life-threatening conditions among patients with hemoglobinopathies. This study investigates B19V infection in patients with sickle cell disease and beta-thalassemia using different experimental approaches. A total of 183 individuals (144 with sickle cell disease and 39 with beta-thalassemia major) and 100 healthy blood donors were examined for B19V using anti-B19V IgG enzyme immunoassay, quantitative PCR, DNA sequencing, and phylogenetic analysis. Viremia was documented in 18.6% of patients and 1% of donors, and was generally characterized by low viral load (VL); however, acute infections were also observed. Anti-B19V IgG was detected in 65.9% of patients with sickle cell disease and in 60% of donors, whereas the patients with thalassemia exhibited relatively low seroreactivity. The seroprevalence varied among the different age groups. In patients, it progressively increased with age, whereas in donors it reached a plateau. Based on partial NS1 fragments, all isolates detected were classified as subgenotype 1A with a tendency to elicit genetically complex infections. Interestingly, quasispecies occurred in the plasma of not only patients but also donors with even higher heterogeneity. The partial NS1 sequence examined did not exhibit positive selection. Quantitation of B19V with a conservative probe is a technically and practically useful approach. The extensive spread of B19V subgenotype 1A in patients and donors and its recent introduction into the countryside of the Sao Paulo State, Brazil were demonstrated; however, it is difficult to establish a relationship between viral sequences and the clinical outcomes of the infection. J. Med. Virol. 84:16521665, 2012. (c) 2012 Wiley Periodicals, Inc. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Instituto Nacional de Ciencia e Tecnologia em Celulas Tronco e Terapia Celular (INCTC) Instituto Nacional de Ciencia e Tecnologia em Celulas Tronco e Terapia Celular (INCTC) |
Identificador |
JOURNAL OF MEDICAL VIROLOGY, v. 84, n. 10, pp. 1652-1665, OCT, 2012 0146-6615 http://www.producao.usp.br/handle/BDPI/33490 10.1002/jmv.23358 |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL HOBOKEN |
Relação |
JOURNAL OF MEDICAL VIROLOGY |
Direitos |
closedAccess Copyright WILEY-BLACKWELL |
Palavras-Chave | #APLASTIC CRISIS #VIRAL LOAD #GENOTYPING #SUBGENOTYPE 1A #MOLECULAR CLOCK #ORGANIZATION INTERNATIONAL STANDARD #ACUTE SPLENIC SEQUESTRATION #POLYMERASE-CHAIN-REACTION #GENETIC DIVERSITY #APLASTIC CRISIS #BONE-MARROW #CLINICAL PRESENTATIONS #HUMAN ERYTHROVIRUSES #3 GENOTYPES #INFECTION #VIROLOGY |
Tipo |
article original article publishedVersion |