Hydrogen production and consumption of organic acids by a phototrophic microbial consortium
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
---|---|
Data(s) |
04/11/2013
04/11/2013
2012
|
Resumo |
Alternative fuel sources have been extensively studied. Hydrogen gas has gained attention because its combustion releases only water, and it can be produced by microorganisms using organic acids as substrates. The aim of this study was to enrich a microbial consortium of photosynthetic purple non-sulfur bacteria from an Upflow Anaerobic Sludge Blanket reactor (UASB) using malate as carbon source. After the enrichment phase, other carbon sources were tested, such as acetate (30 mmol l(-1)), butyrate (17 mmol l(-1)), citrate (11 mmol l(-1)), lactate (23 mmol l(-1)) and malate (14.5 mmol l(-1)). The reactors were incubated at 30 degrees C under constant illumination by 3 fluorescent lamps (81 mu mol m(-2) s(-1)). The cumulative hydrogen production was 7.8, 9.0, 7.9, 5.6 and 13.9 mmol H-2 l(-1) culture for acetate, butyrate, citrate, lactate and malate, respectively. The maximum hydrogen yield was 0.6, 1.4, 0.7, 0.5 and 0.9 mmol H-2 mmol(-1) substrate for acetate, butyrate, citrate, lactate and malate, respectively. The consumption of substrates was 43% for acetate, 37% for butyrate, 100% for citrate, 49% for lactate and 100% for malate. Approximately 26% of the clones obtained from the Phototrophic Hydrogen-Producing Bacterial Consortium (PHPBC) were similar to Rhodobacter, Rhodospirillum and Rhodopseudomonas, which have been widely cited in studies of photobiological hydrogen production. Clones similar to the genus Sulfurospirillum (29% of the total) were also found in the microbial consortium. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. Sao Paulo Research Foundation (FAPESP) Sao Paulo Research Foundation (FAPESP) National Council for Scientific and Technological Development (CNPq) [131296/2007-8] National Council for Scientific and Technological Development (CNPq) |
Identificador |
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, OXFORD, v. 37, n. 16, supl. 1, Part 3, pp. 11691-11700, AUG, 2012 0360-3199 http://www.producao.usp.br/handle/BDPI/37914 10.1016/j.ijhydene.2012.05.088 |
Idioma(s) |
eng |
Publicador |
PERGAMON-ELSEVIER SCIENCE LTD OXFORD |
Relação |
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY |
Direitos |
closedAccess Copyright PERGAMON-ELSEVIER SCIENCE LTD |
Palavras-Chave | #MIXED CULTURE #RHODOBACTER #SULFUROSPIRILLUM #ORGANIC ACIDS #ANAEROBIC BATCH REACTORS #RHODOBACTER-SPHAEROIDES OU001 #MILL WASTE-WATER #SP-NOV #H-2 PRODUCTION #ACETATE #FERMENTATION #BUTYRATE #PHOTOPRODUCTION #GROWTH #MUTANT #CHEMISTRY, PHYSICAL #ELECTROCHEMISTRY #ENERGY & FUELS |
Tipo |
article original article publishedVersion |