Time sequence of the intensification of the liver glucose production induced by high-fat diet in mice
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
04/11/2013
04/11/2013
2012
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Resumo |
It is well established that the development of insulin resistance shows a temporal sequence in different organs and tissues. Moreover, considering that the main aspect of insulin resistance in liver is a process of glucose overproduction from gluconeogenesis, we investigated if this metabolic change also shows temporal sequence. For this purpose, a well-established experimental model of insulin resistance induced by high-fat diet (HFD) was used. The mice received HFD (HFD group) or standard diet (COG group) for 1, 7, 14 or 56?days. The HFD group showed increased (P?<?0.05 versus COG) epididymal, retroperitoneal and inguinal fat weight from days 1 to 56. In agreement with these results, the HFD group also showed higher body weight (P?<?0.05 versus COG) from days 7 to 56. Moreover, the changes induced by HFD on liver gluconeogenesis were progressive because the increment (P?<?0.05 versus COG) in glucose production from l-lactate, glycerol, l-alanine and l-glutamine occurred 7, 14, 56 and 56 days after the introduction of the HFD schedule, respectively. Furthermore, glycaemia and cholesterolemia increased (P?<?0.05 versus COG) 14?days after starting the HFD schedule. Taken together, the results suggest that the intensification of liver gluconeogenesis induced by an HFD is not a synchronous all-or-nothing process but is specific for each gluconeogenic substrate and is integrated in a temporal manner with the progressive augmentation of fasting glycaemia. Copyright (c) 2012 John Wiley & Sons, Ltd. CNPq CNPq [563870/2010-9] FAPESP FAPESP Araucaria Foundation Araucaria Foundation CAPES CAPES |
Identificador |
CELL BIOCHEMISTRY AND FUNCTION, HOBOKEN, v. 30, n. 4, supl. 4, Part 1-2, pp. 335-339, JUN, 2012 0263-6484 http://www.producao.usp.br/handle/BDPI/37780 10.1002/cbf.2809 |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL HOBOKEN |
Relação |
CELL BIOCHEMISTRY AND FUNCTION |
Direitos |
closedAccess Copyright WILEY-BLACKWELL |
Palavras-Chave | #LIVER GLUCONEOGENESIS #HYPERGLYCAEMIA #HIGH-FAT DIET #INSULIN RESISTANCE #HEPATIC INSULIN-RESISTANCE #INDUCED HYPOGLYCEMIA #METABOLIC SYNDROME #INDUCED OBESITY #FED RATS #DISEASE #BIOCHEMISTRY & MOLECULAR BIOLOGY #CELL BIOLOGY |
Tipo |
article original article publishedVersion |