Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)


Autoria(s): Biller-Takahashi, Jaqueline Dalbello; Takahashi, Leonardo Susumu; Machado, Cleni Mara Marzocchi; Zanuzzo, Fabio Sabbadin; Sabioni, Rafael Estevan; Urbinati, Elisabeth Criscuolo
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

04/11/2013

04/11/2013

02/08/2013

Resumo

The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of beta-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% beta-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the hemolytic activity of the proteins of the complement system (alternative pathway) increased after the pathogen challenge, but was not influenced by the beta-glucan treatment.

Identificador

REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, VICOSA, v. 41, n. 2, pp. 237-241, FEB, 2012

1516-3598

http://www.producao.usp.br/handle/BDPI/37757

10.1590/S1516-35982012000200001

http://dx.doi.org/10.1590/S1516-35982012000200001

Idioma(s)

eng

Publicador

REVISTA BRASILEIRA ZOOTECNIA BRAZILIAN JOURNAL ANIMAL SCI

VICOSA

Relação

REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE

Direitos

openAccess

Copyright REVISTA BRASILEIRA ZOOTECNIA BRAZILIAN JOURNAL ANIMAL SCI

Palavras-Chave #A. HYDROPHILA #BETA-GLUCAN #IMMUNE RESPONSE #PACU #TROUT ONCORHYNCHUS-MYKISS #DIETARY BETA-1,3 GLUCAN #DISEASE RESISTANCE #RAINBOW-TROUT #VITAMIN-C #AEROMONAS-HYDROPHILA #DEFENSE-MECHANISMS #FISH #MACROGARD #RESPONSES #AGRICULTURE, DAIRY & ANIMAL SCIENCE #VETERINARY SCIENCES
Tipo

article

original article

publishedVersion