A Modified Technique of Rat Tail Suspension for Longer Periods of Observation
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
01/11/2013
01/11/2013
2012
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Resumo |
FALCAI MJ, LOUZADA MJQ, DE PAULA FJA, OKUBO R, VOLPON JB. A modified technique of rat tail suspension for longer periods of observation. Aviat Space Environ Med 2012; 83:1176-80. Background: Rat tail suspension is an accepted method to create experimental osteopenia. However, suspension periods longer than 3 wk may cause tail skin sloughing or rat slippage. The hypothesis was that a traction system with skeletal anchorage through one tail vertebra would prolong the suspension time without significant complications. Methods: There were 80 young adult female Wistar rats that were submitted to one of the following interventions: skeletal tail suspension (N = 20), skin tail suspension (N = 20), no intervention (N = 20), and a baseline control (N = 20). All animals were followed up either for 3 (N = 10) or 6 (N = 10) wk. Animals were assessed for clinical signs of stress and tolerance to suspension. The femur evaluation was in terms of mineral density content, mechanical resistance, and histomorphometry. Results/Discussion: All animals reached the 3-wk end point. However, for the 6-wk period, seven animals suspended by the skin traction method were discarded (70%) because of signs of stress and skin sloughing. In contrast, there was one loss in the skeletal suspension group (10%). All suspended animals developed similar osteopenia at 3 wk characterized by decreased bone mineral content, weakened bone resistance, and loss of femoral mass. At 6 wk, all suspended animals had similar osteopenic parameters, but they were not statistically different from those of the rats in the 3-wk groups. Therefore, suspension longer than 3 wk did not increase the bone deterioration in the femur. State of Sao Paulo Research Foundation (FAPESP) State of Sao Paulo Research Foundation (FAPESP) |
Identificador |
AVIATION SPACE AND ENVIRONMENTAL MEDICINE, ALEXANDRIA, v. 83, n. 12, supl., Part 3, pp. 1176-1180, DEC, 2012 0095-6562 http://www.producao.usp.br/handle/BDPI/37599 10.3357/ASEM.3248.2012 |
Idioma(s) |
eng |
Publicador |
AEROSPACE MEDICAL ASSOC ALEXANDRIA |
Relação |
AVIATION SPACE AND ENVIRONMENTAL MEDICINE |
Direitos |
restrictedAccess Copyright AEROSPACE MEDICAL ASSOC |
Palavras-Chave | #SKIN SUSPENSION #SKELETAL SUSPENSION #OSTEOPENIA #HYPOACTIVITY #BONE WEAKENING #BONE #MODEL #WEIGHTLESSNESS #SPACEFLIGHT #PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH #MEDICINE, GENERAL & INTERNAL #SPORT SCIENCES |
Tipo |
article original article publishedVersion |