Establishment of the bacterial fecal community during the first month of life in Brazilian newborns
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
03/10/2013
03/10/2013
2012
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Resumo |
OBJECTIVE: The establishment of the intestinal microbiota in newborns is a critical period with possible long-term consequences for human health. In this research, the development of the fecal microbiota of a group of exclusively breastfed neonates living in low socio-economic conditions in the city of Sao Paulo, Brazil, during the first month of life, was studied. METHODS: Fecal samples were collected from ten neonates on the second, seventh, and 30th days after birth. One of the neonates underwent antibiotic therapy. Molecular techniques were used for analysis; DNA was extracted from the samples, and 16S rRNA libraries were sequenced and phylogenetically analyzed after construction. A real-time polymerase chain reaction (PCR) was performed on the samples taken from the 30th day to amplify DNA from Bifidobacterium sp. RESULTS: The primary phylogenetic groups identified in the samples were Escherichia and Clostridium. Staphylococcus was identified at a low rate. Bifidobacterium sp. was detected in all of the samples collected on the 30th day. In the child who received antibiotics, a reduction in anaerobes and Escherichia, which was associated with an overgrowth of Klebsiella, was observed throughout the experimental period. CONCLUSION: The observed pattern of Escherichia predominance and reduced Staphylococcus colonization is in contrast with the patterns observed in neonates living in developed countries. |
Identificador |
CLINICS, SAO PAULO, v. 67, n. 2, pp. 113-123, MAY 28, 2012 1807-5932 http://www.producao.usp.br/handle/BDPI/34022 10.6061/clinics/2012(02)05 |
Idioma(s) |
eng |
Publicador |
HOSPITAL CLINICAS, UNIV SAO PAULO SAO PAULO |
Relação |
CLINICS |
Direitos |
openAccess Copyright HOSPITAL CLINICAS, UNIV SAO PAULO |
Palavras-Chave | #MICROBIOTA #BREASTFEEDING #16S RRNA #ESCHERICHIA #CLOSTRIDIUM #INTESTINAL MICROBIOTA #ESCHERICHIA-COLI #GUT MICROBIOTA #CLOSTRIDIUM-DIFFICILE #ANTIBIOTIC-TREATMENT #SEQUENCE DATA #FED INFANTS #COLONIZATION #FLORA #CHILDREN #MEDICINE, GENERAL & INTERNAL |
Tipo |
article original article publishedVersion |