Improving the performance of a glucose biosensor using an ionic liquid for enzyme immobilization. On the chemical interaction between the biomolecule, the ionic liquid and the cross-linking agent
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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| Data(s) |
29/10/2013
29/10/2013
2012
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| Resumo |
The effect of the room temperature ionic liquid (1-butyl-2,3-dimethylimidazolium tetrafluoroborate ([BMMI][BF4])) on the immobilization of glucose oxidase (GOx) was studied. The electrochemical performance of biosensors prepared following different protocols indicated a beneficial effect of the ionic liquid on the analytical parameters. The chemical interaction between GOx, [BMMI][BF4] and glutaraldehyde was investigated using UV-visible spectroscopy (UV-vis) and circular dichroism (CD). Structural changes of the biomolecule were observed to depend on the method used for the immobilization. (C) 2011 Elsevier Ltd. All rights reserved. CNPq CNPq [554553/2008-2] FAPESP [09/53199-3] FAPESP INCT of Bionalitica (CNPq/FAPESP/MCT) INCT of Bionalitica (CNPq/FAPESP/MCT) |
| Identificador |
ELECTROCHIMICA ACTA, OXFORD, v. 73, n. 8, pp. 123-128, 37073, 2012 0013-4686 http://www.producao.usp.br/handle/BDPI/36241 10.1016/j.electacta.2011.10.088 |
| Idioma(s) |
eng |
| Publicador |
PERGAMON-ELSEVIER SCIENCE LTD OXFORD |
| Relação |
ELECTROCHIMICA ACTA |
| Direitos |
closedAccess Copyright PERGAMON-ELSEVIER SCIENCE LTD |
| Palavras-Chave | #GLUCOSE OXIDASE #IONIC LIQUID #HYBRID COPPER #HEXACYANOFERRATE/POLYPYRROLE #DIRECT ELECTRON-TRANSFER #COPPER HEXACYANOFERRATE NANOPARTICLES #CARBON NANOTUBE #DIRECT ELECTROCHEMISTRY #ASPERGILLUS-NIGER #REDOX MEDIATOR #OXIDASE #HYBRID #NANOCOMPOSITE #STABILITY #ELECTROCHEMISTRY |
| Tipo |
article original article publishedVersion |
