Crystallization and preliminary X-ray diffraction analysis of endoglucanase III from Trichoderma harzianum
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
23/10/2013
23/10/2013
2012
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Resumo |
Endoglucanases are enzymes that hydrolyze cellulose and are important components of the cellulolytic complex. In contrast to other members of the complex, they cleave internal beta-1,4-glycosidic bonds in the cellulose polymer, allowing cellulose to be used as an energy source. Since biomass is an important renewable source of energy, the structural and functional characterization of these enzymes is of interest. In this study, endoglucanase III from Trichoderma harzianum was produced in Pichia pastoris and purified. Crystals belonging to the orthorhombic space group P212121, with unit-cell parameters a = 47.54, b = 55.57, c = 157.3 angstrom, were obtained by the sitting-drop vapour-diffusion method and an X-ray diffraction data set was collected to 2.07 angstrom resolution. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2009/14333-6, 2008/56255-9, 2009/52840-7] Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) via INCT do Bioetanol Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) via INCT do Bioetanol [471834/2009-2] [490022/2009-0] |
Identificador |
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, MALDEN, v. 68, n. 2, supl. 1, Part 3, pp. 306-309, MAR, 2012 1744-3091 http://www.producao.usp.br/handle/BDPI/35744 10.1107/S1744309112000838 |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL MALDEN |
Relação |
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS |
Direitos |
closedAccess Copyright WILEY-BLACKWELL |
Palavras-Chave | #CELLULOSE #ENDOGLUCANASES #TRICHODERMA HARZIANUM #GLYCOSIDE HYDROLASES #ANGSTROM RESOLUTION #CRYSTAL-STRUCTURES #COMPLEX #CEL12A #REESEI #SUBSTRATE #SUGAR #MODE #BIOCHEMICAL RESEARCH METHODS #BIOCHEMISTRY & MOLECULAR BIOLOGY #BIOPHYSICS #CRYSTALLOGRAPHY |
Tipo |
article original article publishedVersion |