Whole-genome sequencing of the efficient industrial fuel-ethanol fermentative Saccharomyces cerevisiae strain CAT-1


Autoria(s): Babrzadeh, Farbod; Jalili, Roxana; Wang, Chunlin; Shokralla, Shadi; Pierce, Sarah; Robinson-Mosher, Avi; Nyren, Pal; Shafer, Robert W.; Basso, Luiz Carlos; Amorim, Henrique Vianna de; Oliveira, Antonio J.; Davis, Ronald W.; Ronaghi, Mostafa; Gharizadeh, Baback; Stambuk, Boris U.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

12/10/2013

12/10/2013

2012

Resumo

The Saccharomyces cerevisiae strains widely used for industrial fuel-ethanol production have been developed by selection, but their underlying beneficial genetic polymorphisms remain unknown. Here, we report the draft whole-genome sequence of the S. cerevisiae strain CAT-1, which is a dominant fuel-ethanol fermentative strain from the sugarcane industry in Brazil. Our results indicate that strain CAT-1 is a highly heterozygous diploid yeast strain, and the similar to 12-Mb genome of CAT-1, when compared with the reference S228c genome, contains similar to 36,000 homozygous and similar to 30,000 heterozygous single nucleotide polymorphisms, exhibiting an uneven distribution among chromosomes due to large genomic regions of loss of heterozygosity (LOH). In total, 58 % of the 6,652 predicted protein-coding genes of the CAT-1 genome constitute different alleles when compared with the genes present in the reference S288c genome. The CAT-1 genome contains a reduced number of transposable elements, as well as several gene deletions and duplications, especially at telomeric regions, some correlated with several of the physiological characteristics of this industrial fuel-ethanol strain. Phylogenetic analyses revealed that some genes were likely associated with traits important for bioethanol production. Identifying and characterizing the allelic variations controlling traits relevant to industrial fermentation should provide the basis for a forward genetics approach for developing better fermenting yeast strains.

CAPES, Brazil [BEX2793-05-9]

CAPES (Brazil)

Identificador

MOLECULAR GENETICS AND GENOMICS, HEIDELBERG, v. 287, n. 6, supl. 1, Part 1, pp. 485-494, JUN, 2012

1617-4615

http://www.producao.usp.br/handle/BDPI/34204

10.1007/s00438-012-0695-7

http://dx.doi.org/10.1007/s00438-012-0695-7

Idioma(s)

eng

Publicador

SPRINGER HEIDELBERG

HEIDELBERG

Relação

MOLECULAR GENETICS AND GENOMICS

Direitos

closedAccess

Copyright SPRINGER HEIDELBERG

Palavras-Chave #BIOETHANOL #GENOME #SACCHAROMYCES #SUGARCANE #INDUSTRIAL STRAINS #REGULATORY ELEMENTS #BUDDING YEAST #GENE #FAMILY #RESISTANCE #REVEALS #PROTEIN #SUPERFAMILY #DETERMINANT #SENSITIVITY #BIOCHEMISTRY & MOLECULAR BIOLOGY #GENETICS & HEREDITY
Tipo

article

original article

publishedVersion