Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3


Autoria(s): Bigarella, Carolina L.; Vieira Ferro, Karla P.; Barcellos, Karin S. A.; Martins-de-Souza, Daniel; Traina, Fabiola; Novello, Jose C.; Olalla Saad, Sara T.; Archangelo, Leticia Froehlich
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

12/10/2013

12/10/2013

2012

Resumo

ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [101624/2011-5]

Fundacao de Amparo a Pesquisa de Sao Paulo (FAPESP) [09/07322-8, 07/54870-5, 07/08019-1]

Fundacao de Amparo a Pesquisa de Sao Paulo (FAPESP)

Identificador

FEBS LETTERS, AMSTERDAM, v. 586, n. 19, supl. 1, Part 3, pp. 3522-3528, SEP 21, 2012

0014-5793

http://www.producao.usp.br/handle/BDPI/34184

10.1016/j.febslet.2012.08.012

http://dx.doi.org/10.1016/j.febslet.2012.08.012

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

AMSTERDAM

Relação

FEBS LETTERS

Direitos

closedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #ARHGAP21 #PROLIFERATION #SUMO2/3 #POST-TRANSLATIONAL MODIFICATION #SUMOYLATION SITE PREDICTION #RECRUITMENT #CHAINS #BIOCHEMISTRY & MOLECULAR BIOLOGY #BIOPHYSICS #CELL BIOLOGY
Tipo

article

original article

publishedVersion