Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
12/10/2013
12/10/2013
2012
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Resumo |
ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [101624/2011-5] Fundacao de Amparo a Pesquisa de Sao Paulo (FAPESP) [09/07322-8, 07/54870-5, 07/08019-1] Fundacao de Amparo a Pesquisa de Sao Paulo (FAPESP) |
Identificador |
FEBS LETTERS, AMSTERDAM, v. 586, n. 19, supl. 1, Part 3, pp. 3522-3528, SEP 21, 2012 0014-5793 http://www.producao.usp.br/handle/BDPI/34184 10.1016/j.febslet.2012.08.012 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE BV AMSTERDAM |
Relação |
FEBS LETTERS |
Direitos |
closedAccess Copyright ELSEVIER SCIENCE BV |
Palavras-Chave | #ARHGAP21 #PROLIFERATION #SUMO2/3 #POST-TRANSLATIONAL MODIFICATION #SUMOYLATION SITE PREDICTION #RECRUITMENT #CHAINS #BIOCHEMISTRY & MOLECULAR BIOLOGY #BIOPHYSICS #CELL BIOLOGY |
Tipo |
article original article publishedVersion |