Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase


Autoria(s): Dinamarco, Taisa Magnani; Brown, Neil Andrew; Couto de Almeida, Ricardo Sergio; Castro, Patricia Alves de; Savoldi, Marcela; Souza Goldman, Maria Helena de; Goldman, Gustavo Henrique
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

12/09/2013

12/09/2013

2012

Resumo

The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoH(V83F), was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoH(V83F) mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil

Identificador

MICROBES AND INFECTION, AMSTERDAM, v. 14, n. 11, pp. 922-929, SEP, 2012

1286-4579

http://www.producao.usp.br/handle/BDPI/33305

10.1016/j.micinf.2012.05.003

http://dx.doi.org/10.1016/j.micinf.2012.05.003

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

AMSTERDAM

Relação

MICROBES AND INFECTION

Direitos

closedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #ASPERGILLUS FUMIGATUS #CALCINEURIN #NUCLEOSIDE DIPHOSPHATE KINASE #GALLERIA MELLONELLA #NEUROSPORA-CRASSA #STRESS #FUNGI #NIDULANS #POLARITY #DEFECTS #RAM #IMMUNOLOGY #INFECTIOUS DISEASES #MICROBIOLOGY
Tipo

article

original article

publishedVersion