Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
12/09/2013
12/09/2013
2012
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Resumo |
The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoH(V83F), was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoH(V83F) mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil |
Identificador |
MICROBES AND INFECTION, AMSTERDAM, v. 14, n. 11, pp. 922-929, SEP, 2012 1286-4579 http://www.producao.usp.br/handle/BDPI/33305 10.1016/j.micinf.2012.05.003 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE BV AMSTERDAM |
Relação |
MICROBES AND INFECTION |
Direitos |
closedAccess Copyright ELSEVIER SCIENCE BV |
Palavras-Chave | #ASPERGILLUS FUMIGATUS #CALCINEURIN #NUCLEOSIDE DIPHOSPHATE KINASE #GALLERIA MELLONELLA #NEUROSPORA-CRASSA #STRESS #FUNGI #NIDULANS #POLARITY #DEFECTS #RAM #IMMUNOLOGY #INFECTIOUS DISEASES #MICROBIOLOGY |
Tipo |
article original article publishedVersion |