Occurrence of Methanogenic Archaea in Highly Polluted Sediments of Tropical Santos-So Vicente Estuary (So Paulo, Brazil)


Autoria(s): SAIA, Flavia; DOMINGUES, Mercia; PELLIZARI, Vivian; VAZOLLER, Rosana
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2010

Resumo

Little is known about the ability of methanogens to grow and produce methane in estuarine environments. In this study, traditional methods for cultivating strictly anaerobic microorganisms were combined with Fluorescence in situ hybridization (FISH) technique to enrich and identify methanogenic Archaea cultures occurring in highly polluted sediments of tropical Santos-So Vicente Estuary (So Paulo, Brazil). Sediment samples were enriched at 30A degrees C under strict anaerobic and halophilic conditions, using a basal medium containing 2% of sodium chloride and amended with glucose, methanol, and sodium salts of acetate, formate and lactate. High methanogenic activity was detected, as evidenced by the biogas containing 11.5 mmol of methane at 20 days of incubation time and methane yield of 0.138-mmol CH(4)/g organic matter/g volatile suspense solids. Cells of methanogenic Archaea were selected by serial dilution in medium amended separately with sodium acetate, sodium formate, or methanol. FISH analysis revealed the presence of Methanobacteriaceae and Methanosarcina sp. cells.

FAPESP-Fundacao de Amparo a Pesquisa do Estado de Sao Paulo[98/05068-0]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FAPESP-Fundacao de Amparo a Pesquisa do Estado de Sao Paulo[00/08323-3]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificador

CURRENT MICROBIOLOGY, v.60, n.1, p.66-70, 2010

0343-8651

http://producao.usp.br/handle/BDPI/31965

10.1007/s00284-009-9503-y

http://dx.doi.org/10.1007/s00284-009-9503-y

Idioma(s)

eng

Publicador

SPRINGER

Relação

Current Microbiology

Direitos

restrictedAccess

Copyright SPRINGER

Palavras-Chave #MARINE-SEDIMENTS #UK ESTUARY #SAO-PAULO #SP-NOV. #DIVERSITY #DECHLORINATION #PROBES #TETRACHLOROETHYLENE #CONTAMINATION #HYBRIDIZATION #Microbiology
Tipo

article

original article

publishedVersion