Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation


Autoria(s): Lima, Luiz Gustavo Ferraz; Beloti, Carolina Aparecida Carlin; Perri, SÍlvia Helena Venturoli; Kaneto, Carlos Noriyuki; Bresciani, Katia Denise Saraiva; Prado, Ângelo Pires do
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

28/01/2016

28/01/2016

2014

Resumo

Several studies have required Haematobia irritans (L.) raising in laboratory. The present study assessed two methods of inoculating immature forms of H. irritans to obtain adults. In 2007, 15 Nellore steers (Bos indicus) (L.) were used for the collection of feces free of anthelmintic treatment and flies to produce for eggs and larva. For method I, 30 eggs were incubated in square filter paper (5 × 5 cm) and deposited on bovine feces (500 g) where they were kept until hatching and spontaneous penetration of larvae (L1) into the fecal mass. After 24 h, eggs were analyzed under a stereoscope microscope (40×) for the number of larvae that instinctively penetrated the feces. In method II, larvae were obtained only by natural egg hatching. At birth, 30 larvae were collected and individually inoculated, directly onto the fecal plate by employing a moistened brush. The tests were carried out at controlled temperature (28˚C ± 2˚C) and saturated humidity (80%) until the emergence of flies with both methods. The number of emerged flies was considered in the result. Using method I, 276 (76.7%) flies emerged from 360 inoculated eggs, while using method II, 283 (78.6%) flies emerged from 360 inoculated larvae. There was no significant difference (P = 0.7821) between methods for the number of flies; however, the proportion between males and females by means of larva inoculation was different from 1:1 (P = 0.0146). Results indicated that both methods led to a satisfactory production of flies and egg inoculation provided an easier establishment.

Formato

76-80

Identificador

http://dx.doi.org/10.4236/ae.2014.22013

Advances in Entomology, v. 2, n. 2, p. 76-80, 2014.

2331-1991

http://hdl.handle.net/11449/132986

10.4236/ae.2014.22013

ISSN2331-1991-2014-02-02-76-80.pdf

7400466027704614

Idioma(s)

eng

Publicador

Scientific Research

Relação

Advances in Entomology

Direitos

openAccess

Palavras-Chave #Haematobia irritans #Horn Fly #Hatched Egg #Methods #Breeding
Tipo

info:eu-repo/semantics/article