Production and Characterization of β-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31


Autoria(s): Pereira, Josiani de Cassia; Leite, Rodrigo Simões Ribeiro; Prado, Heloiza Ferreira Alves do; Martins, Daniela Alonso Bocchini; Gomes, Eleni; Silva, Roberto da
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

15/05/2015

15/05/2015

2014

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

In this paper, several agro-industrial wastes (soybean meal and wheat straw, rice and peanut husks, corn cob and corn stover, and sugarcane bagasse) were tested for the production of β-glucosidase by the cultivation of thermophilic fungus Thermomucor indicae-seudaticae N31 in solid-state fermentation (SSF). Among the tested substrates, the highest yields were obtained in soybean meal. Other fermentation parameters were also evaluated, such as initial pH, merge substrates, and fermentation time, as well as the physicochemical characterization of the enzyme. The best results were obtained after 192 h of fermentation with the initial pH adjusted to 6.0. The substrate mixture did not improve the enzyme production by microorganism. The β-glucosidase showed best catalytic activity at pH 4.5 and at 75 °C and remained stable in the pH range from 4.5 to 9.5 and the temperature range 40–75 °C. The enzyme showed 80 % of its activity at a concentration of 15 mM glucose and remained stable up to 20 % ethanol.

Formato

723-732

Identificador

http://link.springer.com/article/10.1007%2Fs12010-014-1332-1

Applied Biochemistry and Biotechnology, v. 175, p. 723-732, 2014.

0273-2289

http://hdl.handle.net/11449/132299

http://dx.doi.org/10.1007/s12010-014-1332-1

0211880658331763

7091241742851920

9424175688206545

7887148115540704

Idioma(s)

eng

Relação

Applied Biochemistry and Biotechnology

Direitos

closedAccess

Palavras-Chave #Thermomucor indicae-seudaticae N31 #β-glucosidase #Solid-state fermentation
Tipo

info:eu-repo/semantics/article