Production and Characterization of β-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
15/05/2015
15/05/2015
2014
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Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) In this paper, several agro-industrial wastes (soybean meal and wheat straw, rice and peanut husks, corn cob and corn stover, and sugarcane bagasse) were tested for the production of β-glucosidase by the cultivation of thermophilic fungus Thermomucor indicae-seudaticae N31 in solid-state fermentation (SSF). Among the tested substrates, the highest yields were obtained in soybean meal. Other fermentation parameters were also evaluated, such as initial pH, merge substrates, and fermentation time, as well as the physicochemical characterization of the enzyme. The best results were obtained after 192 h of fermentation with the initial pH adjusted to 6.0. The substrate mixture did not improve the enzyme production by microorganism. The β-glucosidase showed best catalytic activity at pH 4.5 and at 75 °C and remained stable in the pH range from 4.5 to 9.5 and the temperature range 40–75 °C. The enzyme showed 80 % of its activity at a concentration of 15 mM glucose and remained stable up to 20 % ethanol. |
Formato |
723-732 |
Identificador |
http://link.springer.com/article/10.1007%2Fs12010-014-1332-1 Applied Biochemistry and Biotechnology, v. 175, p. 723-732, 2014. 0273-2289 http://hdl.handle.net/11449/132299 http://dx.doi.org/10.1007/s12010-014-1332-1 0211880658331763 7091241742851920 9424175688206545 7887148115540704 |
Idioma(s) |
eng |
Relação |
Applied Biochemistry and Biotechnology |
Direitos |
closedAccess |
Palavras-Chave | #Thermomucor indicae-seudaticae N31 #β-glucosidase #Solid-state fermentation |
Tipo |
info:eu-repo/semantics/article |