Development, characterization, and in vitro biological performance of fluconazole-loaded microemulsions for the topical treatment of cutaneous leishmaniasis
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
21/10/2015
21/10/2015
01/01/2015
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Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Cutaneous leishmaniasis (CL) is a resistant form of leishmaniasis that is caused by a parasite belonging to the genus Leishmania. FLU-loaded microemulsions (MEs) were developed by phase diagram for topical administration of fluconazole (FLU) as prominent alternative to combat CL. Three MEs called F1, F2, and F3 (F1-60% 50 M phosphate buffer at pH 7.4 (PB) as aqueous phase, 10% cholesterol (CHO) as oil phase, and 30% soy phosphatidylcholine/oil polyoxyl-60 hydrogenated castor oil/sodium oleate (3/8/6) (S) as surfactant; F2-50% PB, 10% CHO, and 40% S; F3-40% PB, 10% CHO, and 50 % S) were characterized by droplet size analysis, zeta potential analysis, X-ray diffraction, continuous flow, texture profile analysis, and in vitro bioadhesion. MEs presented pseudoplastic flow and thixotropy was dependent on surfactant concentration. Droplet size was not affected by FLU. FLU-loaded MEs improved the FLU safety profile that was evaluated using red cell haemolysis and in vitro cytotoxicity assays with J-774 mouse macrophages. FLU-unloaded MEs did not exhibit leishmanicidal activity that was performed using MTT colourimetric assays; however, FLU-loaded MEs exhibited activity. Therefore, these MEs have potential to modulate FLU action, being a promising platform for drug delivery systems to treat CL. |
Formato |
1-12 |
Identificador |
http://www.hindawi.com/journals/bmri/2015/396894/ Biomed Research International. New York: Hindawi Publishing Corporation, v. 2015, p. 1-12, 2015. 2314-6133 http://hdl.handle.net/11449/129394 http://dx.doi.org/10.1155/2015/396894 WOS:000348787400001 WOS000348787400001.pdf |
Idioma(s) |
eng |
Publicador |
Hindawi Publishing Corporation |
Relação |
Biomed Research International |
Direitos |
openAccess |
Tipo |
info:eu-repo/semantics/article |