Biomodulation of inflammatory cytokines related to oral mucositis by low-level laser therapy


Autoria(s): Basso, Fernanda G.; Pansani, Taisa N.; Soares, Diana G.; Scheffel, Débora L.; Bagnato, Vanderlei S.; Costa, Carlos Alberto de Souza; Hebling, Josimeri
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

06/08/2015

06/08/2015

2015

Resumo

This study evaluated the effects of LLLT on the expressionof inflammatory cytokines related to the development of oralmucositis by gingival fibroblasts. Primary gingival fibroblastswere seeded on 24-well plates (105cells/well) for 24 h. Freshserum-free culture medium (DMEM) was then added, andcells were placed in contact with LPS (Escherichia coli,1 lgmL1), followed by LLLT irradiation (LaserTABLE—InGaAsP diode prototype—780 nm, 25 mW) delivering 0,0.5, 1.5 or 3 J cm². Cells without contact with LPS werealso irradiated with the same energy densities. Gene expres-sion of TNF- a, IL-1b, IL-6 and IL-8 was evaluated by Real-Time PCR, and protein synthesis of these cytokines wasdetermined by enzyme-linked immunosorbent (ELISA) assay.Data were statistically analyzed by the Kruskal– Wallis test,complemented by the Mann–Whitney test (P < 0.05). LPStreatment increased the gene expression and protein synthesisof TNF-a, IL-6 and IL-8, while the expression of IL-1b wasnot affected. For LPS-treated groups, LLLT promoted signif-icant decreases in the expression of TNF-a, IL-6, and IL-8 at1.5 J cm2and 3 J cm2. These results demonstrate thatLLLT promoted a beneficial biomodulatory effect on theexpression of inflammatory cytokines related to oral mucosi-tis by human gingival fibroblasts.

Formato

952-956

Identificador

http://onlinelibrary.wiley.com/doi/10.1111/php.12445/abstract

Photochemistry and Photobiology, v. 91, n. 4, p. 952-956, 2015.

0031-8655

http://hdl.handle.net/11449/125538

http://dx.doi.org/10.1111/php.12445

8207097271172991

4517484241515548

Idioma(s)

eng

Relação

Photochemistry and Photobiology

Direitos

closedAccess

Tipo

info:eu-repo/semantics/article