Isolation and Identification of Environmental Mycobacteria in the Waters of a Hemodialysis Center
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
---|---|
Data(s) |
03/12/2014
03/12/2014
01/07/2013
|
Resumo |
The use of poorly treated water during hemodialysis may lead to contamination with nontuberculous mycobacteria (NTM). This study aimed to isolate and identify NTM species in the water of a Brazilian hemodialysis center. We collected 210 samples of water from the hydric system of the unit (post-osmosis system, hemodialysis rooms, reuse system, and hemodialysis equipment) and from the municipal supply network; we isolated the NTM by a classic microbiological technique and identified them by the PCR restriction enzyme pattern of the hsp65 gene (PRA). Fifty-one (24.3 %) of the collected samples tested positive for NTM; both the municipal supply network (2 samples, 3.2 %) and the hydric system of the hemodialysis center (49 samples, 96.1 %) contained NTM. We isolated and identified potentially pathogenic bacteria such as Mycobacterium lentiflavum (59.0 %) and M. kansasii (5.0 %), as well as rarely pathogenic bacteria like M. gordonae (24.0 %), M. gastri (8.0 %), and M. szulgai (4.0 %). The ability of NTM to cause diseases is well documented in the literature. Therefore, the identification of NTM in the water of a Brazilian hemodialysis center calls for more effective water disinfection procedures in this unit. |
Formato |
107-111 |
Identificador |
http://dx.doi.org/10.1007/s00284-013-0341-6 Current Microbiology. New York: Springer, v. 67, n. 1, p. 107-111, 2013. 0343-8651 http://hdl.handle.net/11449/113451 10.1007/s00284-013-0341-6 WOS:000319361100014 |
Idioma(s) |
eng |
Publicador |
Springer |
Relação |
Current Microbiology |
Direitos |
closedAccess |
Tipo |
info:eu-repo/semantics/article |