Label-free Capacitive Diagnostics: Exploiting Local Redox Probe State Occupancy


Autoria(s): Lehr, Joshua; Bedatty Fernandes, Flavio C.; Bueno, Paulo Roberto; Davis, Jason J.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

03/12/2014

03/12/2014

04/03/2014

Resumo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

An electrode surface confined redox group contributes to a substantial potential-dependent interfacial charging that can be sensitively probed and frequency-resolved by impedance-derived capacitance spectroscopy. In utilizing the sensitivity of this charging fingerprint to redox group environment, one can seek to generate derived sensory configurations. Exemplified here through the generation of mixed molecular films comprising ferrocene and antibody receptors to two clinically important targets, the label-free methodology is able to report on human prostatic acid phosphatase (PAP), a tumor marker, with a limit of detection of 11 pM and C-reactive protein with a limit of detection of 28 pM. Both assays exhibit linear ranges encompassing those of clinical value.

Formato

2559-2564

Identificador

http://dx.doi.org/10.1021/ac403727h

Analytical Chemistry. Washington: Amer Chemical Soc, v. 86, n. 5, p. 2559-2564, 2014.

0003-2700

http://hdl.handle.net/11449/113164

10.1021/ac403727h

WOS:000332494100040

Idioma(s)

eng

Publicador

Amer Chemical Soc

Relação

Analytical Chemistry

Direitos

closedAccess

Tipo

info:eu-repo/semantics/article