Elapid Snake Venom Analyses Show the Specificity of the Peptide Composition at the Level of Genera Naja and Notechis


Autoria(s): Munawar, Aisha; Trusch, Maria; Georgieva, Dessislava; Hildebrand, Diana; Kwiatkowski, Marcel; Behnken, Henning; Harder, Soenke; Arni, Raghuvir; Spencer, Patrick; Schlueter, Hartmut; Betzel, Christian
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

03/12/2014

03/12/2014

01/03/2014

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Elapid snake venom is a highly valuable, but till now mainly unexplored, source of pharmacologically important peptides. We analyzed the peptide fractions with molecular masses up to 10 kDa of two elapid snake venoms-that of the African cobra, N. m. mossambica (genus Naja), and the Peninsula tiger snake, N. scutatus, from Kangaroo Island (genus Notechis). A combination of chromatographic methods was used to isolate the peptides, which were characterized by combining complimentary mass spectrometric techniques. Comparative analysis of the peptide compositions of two venoms showed specificity at the genus level. Three-finger (3-F) cytotoxins, bradykinin-potentiating peptides (BPPs) and a bradykinin inhibitor were isolated from the Naja venom. 3-F neurotoxins, Kunitz/basic pancreatic trypsin inhibitor (BPTI)-type inhibitors and a natriuretic peptide were identified in the N. venom. The inhibiting activity of the peptides was confirmed in vitro with a selected array of proteases. Cytotoxin 1 (P01467) from the Naja venom might be involved in the disturbance of cellular processes by inhibiting the cell 20S-proteasome. A high degree of similarity between BPPs from elapid and viperid snake venoms was observed, suggesting that these molecules play a key role in snake venoms and also indicating that these peptides were recruited into the snake venom prior to the evolutionary divergence of the snakes.

Formato

850-868

Identificador

http://dx.doi.org/10.3390/toxins6030850

Toxins. Basel: Mdpi Ag, v. 6, n. 3, p. 850-868, 2014.

2072-6651

http://hdl.handle.net/11449/112895

10.3390/toxins6030850

WOS:000335755700005

WOS000335755700005.pdf

Idioma(s)

eng

Publicador

Mdpi Ag

Relação

Toxins

Direitos

openAccess

Palavras-Chave #natriuretic peptides #Notechis scutatus from Kangaroo Island #Naja mossambica mossambica #Snake venom #pharmacologically active peptides #Bradykinin potentiating peptides #Kunitz-type inhibitor #neurotoxin #cytotoxin
Tipo

info:eu-repo/semantics/article