Easily handling penicillin G acylase magnetic cross-linked enzymes aggregates: Catalytic and morphological studies
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
03/12/2014
03/12/2014
01/01/2014
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Biomolecules labeled with superparamagnetic nanoparticles can be selectively removed from complex reaction mixtures using an external magnetic field. Amino-functionalized superparamagnetic iron oxide nanoparticles (amino-SPION) were co-aggregated with penicillin G acylase and then cross-linked, generating magnetic cross-linked enzymes aggregates (M-CLEAs) that were quickly and efficiently recovered from the reaction medium by applying an external magnetic field. M-CLEAs and cross-linked enzymes aggregates (CLEAs) prepared under the same reaction conditions were characterized and compared. The best recovered activities were obtained for M-CLEAs prepared using polyethylene glycol 600 as precipitant and the most stable M-CLEA were obtained using tert-butanol. Successive penicillin G hydrolysis reactions were carried out using the same M-CLEA in a 50 mL reactor (3 reaction cycles), after the reactions the derivate was magnetically recovered without loss of activity demonstrating a total magnetic recovery. Line-scan energy dispersive X-ray spectroscopy showed that the amino-SPIONs were homogeneously dispersed within the structure of the M-CLEA. (C) 2013 Elsevier Ltd. All rights reserved. |
Formato |
38-46 |
Identificador |
http://dx.doi.org/10.1016/j.procbio.2013.09.024 Process Biochemistry. Oxford: Elsevier Sci Ltd, v. 49, n. 1, p. 38-46, 2014. 1359-5113 http://hdl.handle.net/11449/111697 10.1016/j.procbio.2013.09.024 WOS:000331156100006 |
Idioma(s) |
eng |
Publicador |
Elsevier B.V. |
Relação |
Process Biochemistry |
Direitos |
closedAccess |
Palavras-Chave | #Cross-linked enzymes aggregates #Superparamagnetic nanoparticles #Supramolecular complex #Magnetic recovery of enzymes #Penicillin hydrolysis |
Tipo |
info:eu-repo/semantics/article |