Penicillinase-based amperometric biosensor for penicillin G
| Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
|---|---|
| Data(s) |
03/12/2014
03/12/2014
01/01/2014
|
| Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved. |
| Formato |
131-133 |
| Identificador |
http://dx.doi.org/10.1016/j.elecom.2013.11.022 Electrochemistry Communications. New York: Elsevier Science Inc, v. 38, p. 131-133, 2014. 1388-2481 http://hdl.handle.net/11449/111420 10.1016/j.elecom.2013.11.022 WOS:000331504300035 |
| Idioma(s) |
eng |
| Publicador |
Elsevier B.V. |
| Relação |
Electrochemistry Communications |
| Direitos |
closedAccess |
| Palavras-Chave | #Cysteine #Metallo-beta-lactamase #Self-assembled monolayer (SAM) #beta-Lactam antibiotic #Lineweaver-Burk plot |
| Tipo |
info:eu-repo/semantics/article |
