Histologic Characterization of Engineered Tissues in the Canal Space of Closed-apex Teeth with Apical Periodontitis
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
27/05/2014
27/05/2014
30/09/2013
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Resumo |
Introduction: The aim of this study was to investigate the capacity of endodontic regenerative procedures combining an induced blood clot, platelet-rich plasma (PRP), and bone marrow aspirate (BMA) to regenerate dental pulp in canine closed-apex necrotic teeth. Methods: Apical periodontitis was induced in 20 upper and lower premolars of 2 dogs. After biomechanical preparation, enlargement to a #60 file, and disinfection with a triantibiotic paste for 28 days, the roots were randomly assigned to 4 treatment groups: blood clot (BC), BC + PRP gel, BC + BMA gel, and BC + BMA/PRP gel. Negative controls were also included. After a 3-month follow-up period, the animals were killed. Results: Histologic analysis showed the presence of newly formed vital tissues (connective, cement-like, and bone-like tissue) in 23 of the 32 treated roots (71.87%). There was no statistically significant difference between the treatment groups. Conclusions: New vital tissues were formed and characterized as connective, cementum-like, or bone-like, but not as pulp-like tissue; PRP and/or BMA did not improve the tissue ingrowth. © 2013 American Association of Endodontists. |
Identificador |
http://dx.doi.org/10.1016/j.joen.2013.08.023 Journal of Endodontics. 0099-2399 http://hdl.handle.net/11449/76661 10.1016/j.joen.2013.08.023 WOS:000327916700014 2-s2.0-84884538597 |
Idioma(s) |
eng |
Relação |
Journal of Endodontics |
Direitos |
closedAccess |
Palavras-Chave | #Bone marrow aspirate #necrotic teeth #platelet-rich plasma #regenerative endodontics #tissue engineering #vital tissue growth |
Tipo |
info:eu-repo/semantics/article |