New label free CA125 detection based on gold nanostructured screen-printed electrode


Autoria(s): Ravalli, A.; Pilon Dos Santos, G.; Ferroni, M.; Faglia, G.; Yamanaka, Hideko; Marrazza, G.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

01/01/2013

Resumo

This paper describes the optimisation and the analytical performances of a label-free impedimetric immunosensor for the detection of tumour marker CA125 based on gold nanoparticles modified screen-printed graphite electrode. Experimental conditions of each step for the developed immunosensor were studied and optimised. The immunosensor response varied linearly (r2 = 0.996) with antigen concentration between 0 and 100 U/mL. The estimated detection limit was 6.7 U/mL. The electrochemical immunosensor allowed unambiguous identification of CA125, while no significant non-specific signal was detected in the case of all negative controls. The analytical usefulness of the impedimetric immunosensor was finally demonstrated analysing serum samples. © 2012 Elsevier B.V. All rights reserved.

Formato

194-200

Identificador

http://dx.doi.org/10.1016/j.snb.2012.10.017

Sensors and Actuators, B: Chemical, v. 179, p. 194-200.

0925-4005

http://hdl.handle.net/11449/74197

10.1016/j.snb.2012.10.017

WOS:000316588100024

2-s2.0-84880921300

Idioma(s)

eng

Relação

Sensors and Actuators B: Chemical

Direitos

closedAccess

Palavras-Chave #CA125 #Gold nanoparticles #Impedimetric immunosensor #Screen-printed electrode #Tumour marker #Analytical performance #Detection limits #Electrochemical immunosensors #Experimental conditions #Gold Nanoparticles #Impedimetric immunosensors #Screen printed electrodes #Gold #Graphite electrodes #Metal nanoparticles #Tumors #Immunosensors
Tipo

info:eu-repo/semantics/article