Anticlastogenic effect of β-glucan, extracted from Saccharomyces cerevisiae, on cultured cells exposed to ultraviolet radiation


Autoria(s): Da Silva, Ariane Fernanda; Oliveira, Rodrigo Juliano; Niwa, Andressa Megumi; D'Epiro, Gláucia Fernanda Rocha; Ribeiro, Lúcia Regina; Mantovani, Mário Sérgio
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

01/01/2013

Resumo

β-glucan is an important polysaccharide due to its medicinal properties of stimulating the immune system and preventing chronic diseases such as cancer. The aim of the present study was to determine the anticlastogenic effect of β-glucan in cells exposed to ultraviolet radiation (UV). Chromosome aberration assay was performed in drug-metabolizing cells (HTC) and non drug-metabolizing cells (CHO-K1 and repair-deficient CHO-xrs5), using different treatment protocols. Continuous treatment (UV + β-glucan) was not effective in reducing the DNA damage only in CHO-xrs5 cells. However, the pre-treatment protocol (β-glucan before UV exposition) was effective in reducing DNA damage only in CHO-K1 cells. In post-treatment (β-glucan after UV exposition) did not show significative anticlastogenic effects, although there was a tendency toward prevention. The data suggest that β-glucan has more than one action mechanism, being capable of exerting desmutagenic as well as bio-antimutagenic action. The findings also suggest that the presence of the xenobiotic metabolizing system can reduce the chemopreventive capacity of β-glucan. Therefore, these results indicate that β-glucan from Saccharomyces cerevisiae can be used in the prevention and/or reduction of DNA damage. © 2012 Springer Science+Business Media B.V.

Formato

41-48

Identificador

http://dx.doi.org/10.1007/s10616-012-9448-z

Cytotechnology, v. 65, n. 1, p. 41-48, 2013.

0920-9069

1573-0778

http://hdl.handle.net/11449/74132

10.1007/s10616-012-9448-z

WOS:000313074500005

2-s2.0-84872319164

Idioma(s)

eng

Relação

Cytotechnology

Direitos

closedAccess

Palavras-Chave #β-Glucan #Anticlastogenicity #Cell culture #Ultraviolet radiation #antimutagenic agent #beta glucan #cell culture #chemoprophylaxis #chromosome aberration #controlled study #DNA damage #drug mechanism #human #human cell #radiation exposure #Saccharomyces cerevisiae #ultraviolet radiation
Tipo

info:eu-repo/semantics/article