Characterization and in vitro cytocompatibility of an acid-etched titanium surface
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
27/05/2014
27/05/2014
01/01/2010
|
Resumo |
The aims of this study were to characterize the microstructure of a commercially pure titanium (cpTi) surface etched with HCl/H 2SO 4 (AE-cpTi) and to investigate its in vitro cytocompatibility compared to turned cpTi (T-cpTi). T-cpTi showed a grooved surface and AE-cpTi revealed a surface characterized by the presence of micropits. Surface parameters indicated that the AE-cpTi surface is more isotropic and present a greater area compared to T-cpTi. The oxide film thickness was similar between both surfaces; however, AE-cpTi presented more Ti and O and less C. Osteoblastic cell proliferation, alkaline phosphatase activity, and bone-like nodule formation were greater on T-cpTi than on AE-cpTi. These results show that acid etching treatment produced a surface with different topographical and chemical features compared to the turned one, and such surface modification affected negatively the in vitro cytocompatibility of cpTi as demonstrated by decreasing culture growth and expression of osteoblastic phenotype. |
Formato |
3-11 |
Identificador |
http://dx.doi.org/10.1590/S0103-64402010000100001 Brazilian Dental Journal, v. 21, n. 1, p. 3-11, 2010. 0103-6440 1806-4760 http://hdl.handle.net/11449/71517 10.1590/S0103-64402010000100001 S0103-64402010000100001 2-s2.0-77955612098 2-s2.0-77955612098.pdf |
Idioma(s) |
eng |
Relação |
Brazilian Dental Journal |
Direitos |
openAccess |
Palavras-Chave | #Cell culture #Cytocompatibility #Osteoblast #Surface treatment #Titanium #alkaline phosphatase #biological marker #biomaterial #biomedical and dental materials #carbon #hydrochloric acid #sulfuric acid #titanium #titanium dioxide #bone development #cell adhesion #cell culture #cell division #cell proliferation #cell survival #chemistry #comparative study #cytology #dental acid etching #drug effect #human #interferometry #jaw #materials testing #osteoblast #phenotype #scanning electron microscopy #surface property #X ray photoelectron spectroscopy #Acid Etching, Dental #Alkaline Phosphatase #Alveolar Process #Biocompatible Materials #Biological Markers #Carbon #Cell Adhesion #Cell Division #Cell Proliferation #Cell Survival #Cells, Cultured #Dental Materials #Humans #Hydrochloric Acid #Interferometry #Materials Testing #Microscopy, Electron, Scanning #Osteoblasts #Osteogenesis #Phenotype #Photoelectron Spectroscopy #Sulfuric Acids #Surface Properties |
Tipo |
info:eu-repo/semantics/article |