The Rv1712 locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially Phosphorylates dCMP
| Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
|---|---|
| Data(s) |
27/05/2014
27/05/2014
01/04/2009
|
| Resumo |
The Mycobacterium tuberculosis cmk gene, predicted to encode a CMP kinase (CMK), was cloned and expressed, and its product was purified to homogeneity. Steady-state kinetics confirmed that M. tuberculosis CMK is a monomer that preferentially phosphorylates CMP and dCMP by a sequential mechanism. A plausible role for CMK is discussed. Copyright © 2009, American Society for Microbiology. All Rights Reserved. |
| Formato |
2884-2887 |
| Identificador |
http://dx.doi.org/10.1128/JB.01337-08 Journal of Bacteriology, v. 191, n. 8, p. 2884-2887, 2009. 0021-9193 http://hdl.handle.net/11449/70966 10.1128/JB.01337-08 2-s2.0-65249143233 2-s2.0-65249143233.pdf |
| Idioma(s) |
eng |
| Relação |
Journal of Bacteriology |
| Direitos |
closedAccess |
| Palavras-Chave | #cytidine phosphate kinase #deoxycytidine phosphate #monomer #phosphotransferase #unclassified drug #cytidylate kinase #nucleoside monophosphate kinase #recombinant protein #bacterial gene #bacterial strain #controlled study #enzyme kinetics #enzyme phosphorylation #gene expression #gene locus #genetic code #molecular cloning #Mycobacterium tuberculosis #nonhuman #priority journal #protein function #protein purification #steady state #amino acid sequence #enzymology #genetics #isolation and purification #kinetics #metabolism #molecular genetics #phosphorylation #sequence alignment #Amino Acid Sequence #Cloning, Molecular #Deoxycytidine Monophosphate #Gene Expression #Kinetics #Molecular Sequence Data #Nucleoside-Phosphate Kinase #Phosphorylation #Recombinant Proteins #Sequence Alignment |
| Tipo |
info:eu-repo/semantics/article |
