A simple protocol for a low invasive DNA accessing in Stenella longirostris (Cetacea: Delphinidae)


Autoria(s): Farro, Ana Paula C.; Rollo Jr., Mario M.; Silva Jr., José M.; Marino, Celso L.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

01/08/2008

Resumo

The most significant studies about the spinner dolphin (Stenella longirostris) in the Southwestern Atlantic Ocean were conducted in Fernando de Noronha Archipelago, off Northeastern Brazil. The continuity of these studies depends upon the development of non-invasive methods. In this work, we present results from the skin swabbing sampling procedure for this species. We tested the performance of this method for nuclear and mitochondrial DNA analysis, unknown for this population. A total of skin 161 samples were collected during two expeditions. After the contacts the most of the dolphins remained close to the boat. Microsatellites markers and cytochrome b region primers were evaluated and the respective fragments were successfully amplified. Thus, skin swabbing may be considered an efficient strategy to obtain tissue samples for spinner dolphin genetic analysis in Fernando de Noronha Archipelago.

Formato

130-134

Identificador

http://www.panamjas.org/pdf_artigos/PANAMJAS_3(2)_130-134.pdf

Pan-American Journal of Aquatic Sciences, v. 3, n. 2, p. 130-134, 2008.

1809-9009

http://hdl.handle.net/11449/70515

2-s2.0-48249136922

2-s2.0-48249136922.pdf

Idioma(s)

eng

Relação

Pan-American Journal of Aquatic Sciences

Direitos

openAccess

Palavras-Chave #Cytochrome b #Fernando de Noronha Archipelago #Microsatellites #Spinner dolphin #Cetacea #Delphinidae #Stenella (Cetacea) #Stenella longirostris
Tipo

info:eu-repo/semantics/article