Interleukin-8 expression associated with canine mammary tumors
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/01/2011
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Processo FAPESP: 05/51936-0 The use of prognostic markers for mammary cancer is important for routine diagnosis and research. Interleukin-8 (IL-8) is a chemotactic cytokine, produced by several cell types in response to inflammation. The expression, regulation and function of IL-8 in dogs are little known. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. We investigated a possible correlation between IL-8 expression and mammary tumor prognosis in female dogs. IL-8 expression was measured in 50 dogs with mammary neoplasia by immunohistochemistry and real-time PCR. Immunohistochemical staining was done with anti-IL-8 antibodies and PCR amplifications were performed in a 7500 Fast Real-Time PCR system. Gene expression stability was analyzed by the geNorm software. Quantitative real-time PCR showed that IL-8 expression decreased in malignant mammary cells compared to normal mammary tissue, while weak immunostaining was associated with a diagnosis of carcinoma. Complementing earlier studies on IL-8 expression in several types of cancer, including mammary cancer, we conclude that IL-8 has potential for use as a prognostic marker for canine mammary neoplasia. |
Formato |
1522-1532 |
Identificador |
http://dx.doi.org/10.4238/vol10-3gmr1145 Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 10, n. 3, p. 1522-1532, 2011. 1676-5680 http://hdl.handle.net/11449/42605 10.4238/vol10-3gmr1145 WOS:000295823500029 WOS000295823500029.pdf |
Idioma(s) |
eng |
Publicador |
Funpec-editora |
Relação |
Genetics and Molecular Research |
Direitos |
openAccess |
Palavras-Chave | #Interleukin-8 #Mammary cancer #Immunohistochemistry #Real-time PCR #Prognosis |
Tipo |
info:eu-repo/semantics/article |