Molecular and Serologic Detection of Ehrlichia spp. in Endangered Brazilian Wild Captive Felids


Autoria(s): Andre, M. R.; Adania, C. H.; Machado, R. Z.; Allegretti, S. M.; Felippe, P. A. N.; Silva, K. F.; Nakaghi, A. C. H.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/07/2010

Resumo

Ehrlichiosis, an emergent tickborne disease that affects both humans and animals, may represent a threat to the survival and preservation of wild felids in Brazil There are few studies of ehrlichiosis in wild felids in Brazil, but Ehrlichia spp are present in domestic cats Antibodies to Ehrlichia canis have been reported in a puma (Puma concolor) In this study we assessed the presence of these hemoparasites in the blood of Brazilian wild captive felids of the 72 animals tested, 5 (7%) were seropositive for the E cams antigen, and L1 (15%) were positive for E emirs DNA sequences We also performed sequence alignment to establish the identity of the parasite species infecting these animals using 16S rRNA and omp-1 genes Sequences based on 16S rRNA were similar to those found in dogs and cats from Thailand, Brazil, China, and Taiwan and with E canis obtained from a single individual (human) in Venezuela Ehrlichia sp sequence from sampled felines based on omp-1. gene was similar to the p28 and p30 multigene family of E canis To our knowledge, this is the first study of molecular detection of Ehrlichia sp in Brazilian wild feline species

Formato

1017-1023

Identificador

http://www.jwildlifedis.org/content/46/3/1017.abstract

Journal of Wildlife Diseases. Lawrence: Wildlife Disease Assoc, Inc, v. 46, n. 3, p. 1017-1023, 2010.

0090-3558

http://hdl.handle.net/11449/41202

WOS:000280690400043

Idioma(s)

eng

Publicador

Wildlife Disease Assoc, Inc

Relação

Journal of Wildlife Diseases

Direitos

closedAccess

Palavras-Chave #Brazil #Ehrlichia sp #PCR #serology #wild felids
Tipo

info:eu-repo/semantics/article