Alterations of spermatogenesis in etoposide-treated rats: A stereological study


Autoria(s): Freitas, FEL; Cordeiro-Mori, F.; Sasso-Cerri, E.; Lucas, SRR; Miraglia, S. M.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/05/2002

Resumo

The etoposide is an anticancer drug that interacts with topoisomerase II. Thirty-day-old rats received intraperitonially 2mg/kg of etoposide for 30 consecutive days. Their testes were analyzed in the adult phase under light microscopy according to histomorphometric and stereological parameters. Random 3mum-thick-paraplast sections of testis were stained with periodic acid-Schiff reaction and Harris' hematoxylin method. Serum testosterone level and reproductive performance were also investigated. The results showed an accentuated decrease in the frequency of germinal lineage cell types and differentiated spermatogonia were the most affected cell types. Morphometric and stereological testicular parameters exhibited highly, significant reductions in adult etoposide-treated rats. Their reproductive performance diminished but their serum testosterone level was not significantly altered. The mortality frequency of the progenies was 100%.

Formato

227-+

Identificador

http://www.redalyc.org/articulo.oa?id=33906803

Interciencia. Caracas: Interciencia, v. 27, n. 5, p. 227-+, 2002.

0378-1844

http://hdl.handle.net/11449/36964

WOS:000175969700003

WOS000175969700003.pdf

Idioma(s)

eng

Publicador

Interciencia

Relação

Interciencia

Direitos

openAccess

Palavras-Chave #etoposide #seminiferous epithelium #spermatogenesis #testis #topoisomerase
Tipo

info:eu-repo/semantics/article