BINDING OF PARACOCCIDIOIDES-BRASILIENSIS TO LAMININ THROUGH SURFACE GLYCOPROTEIN GP43 LEADS TO ENHANCEMENT OF FUNGAL PATHOGENESIS
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/04/1994
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Resumo |
Extracellular matrix protein laminin binds specifically to yeast forms of Paracoccidioides brasiliensis and enhances adhesion of the fungus to the surface of epithelial Madin-Darby canine kidney cells in vitro. Immunoblotting of fungal extracts showed that the gp43 glycoprotein is responsible for adhesion. This was confirmed by binding assays using purified gp43, with a K-d of 3.7 nM. The coating of P. brasiliensis yeast forms with laminin before injection into hamster testicles enhanced the fungus virulence, resulting in a faster and more severe granulomatous disease. These results indicate that interaction of fungi with extracellular matrix elements may constitute a basis for the evolution of fungal infection toward regional spreading and dissemination. |
Formato |
1465-1469 |
Identificador |
http://iai.asm.org/content/62/4/1465 Infection and Immunity. Washington: Amer Soc Microbiology, v. 62, n. 4, p. 1465-1469, 1994. 0019-9567 http://hdl.handle.net/11449/32339 WOS:A1994NC07400046 WOSA1994NC07400046.pdf |
Idioma(s) |
eng |
Publicador |
Amer Soc Microbiology |
Relação |
Infection and Immunity |
Direitos |
closedAccess |
Tipo |
info:eu-repo/semantics/article |