Determination of Catechin Diastereomers from the Leaves of Byrsonima species Using Chiral HPLC-PAD-CD
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
---|---|
Data(s) |
20/05/2014
20/05/2014
01/08/2010
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) When catechins are found in plant extracts, they are almost always identified as catechin and/or epicatechin probably due to stereoselectivity of the enzymes involved in the biosynthesis of these substances. However, the lack of reports regarding to ent-catechin as well as ent-epicatechin does not necessarily mean that these compounds have not been produced. In fact, most of the previous reports used chromatographic conditions not suitable for such separation. This article describes a simple and reliable analytical HPLC-PAD-CD method for simultaneous determination of catechin diastereomers both in infusions and extracts from the leaves of Byrsonima species. The direct separation of catechin, ent-catechin, epicatechin, and ent-epicatechin was obtained in normal phase by HPLC-PAD-CD using Chiralcel OD-H as chiral stationary phase and n-hexane/ethanol with 0.1% of TFA as mobile phase. Chirality 22: 726-733, 2010. (C) 2010 Wiley-Liss, Inc. |
Formato |
726-733 |
Identificador |
http://dx.doi.org/10.1002/chir.20824 Chirality. Hoboken: Wiley-liss, v. 22, n. 8, p. 726-733, 2010. 0899-0042 http://hdl.handle.net/11449/26246 10.1002/chir.20824 WOS:000280056100003 |
Idioma(s) |
eng |
Publicador |
Wiley-liss |
Relação |
Chirality |
Direitos |
closedAccess |
Palavras-Chave | #chiral chromatography #enantioseparation #circular dichroism #plant extract |
Tipo |
info:eu-repo/semantics/article |